Evidence map›Paper›PMID 27938355›Full record

ArticleBMC veterinary research2016

Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy in combination with environmental dust reduction in equine recurrent airway obstruction.

Ann Kristin Barton, Tarek Shety, Angelika Bondzio, Ralf Einspanier, Heidrun Gehlen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Gene Expression Profiles of the Immuno-Transcriptome in Equine Asthma.Animals : an open access journal from MDPI · 2022
    Article
  6. Review
  7. Glucocorticoid treatment in horses with asthma: A narrative review.Journal of veterinary internal medicine · 2021
    Review
  8. Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ann Kristin BartonEquine Clinic, Freie Universitaet Berlin, Oertzenweg 19b, Berlin, 10163, Germany. Ann-Kristin.Barton@fu-berlin.de.ORCID http://orcid.org/0000-0002-3333-9647
Tarek ShetyEquine Clinic, Freie Universitaet Berlin, Oertzenweg 19b, Berlin, 10163, Germany.
Angelika BondzioInstitute of Veterinary Biochemistry, Freie Universitaet Berlin, Oertzenweg 19b, Berlin, 10163, Germany.
Ralf EinspanierInstitute of Veterinary Biochemistry, Freie Universitaet Berlin, Oertzenweg 19b, Berlin, 10163, Germany.
Heidrun GehlenEquine Clinic, Freie Universitaet Berlin, Oertzenweg 19b, Berlin, 10163, Germany.
Freie Universität Berlin · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOverexpression of matrix-metalloproteinases (MMPs) has been shown to lead to tissue damage in equine recurrent airway obstruction (RAO), as a misbalance with their natural inhibitors, the tissue inhibitors of metalloproteinases (TIMPs), occurs. This favors irreversible pulmonary fibrosis formation. Increased levels of MMPs, TIMPs or altered ratios between them can be used as biomarkers of respiratory disease. We hypothesized that levels of MMPs, TIMPs and their ratios correlate with improvement in clinical findings and bronchoalveolar lavage fluid (BALF) cytology after 10 days of inhalative glucocorticoid therapy and environmental dust reduction (EDR) and may be used to monitor treatment success. Ten horses with a history of RAO participated in a prospective clinical study. Clinical and cytological scoring was performed before and after inhalative therapy using budesonide (1500 μg BID over 10 days) and EDR (bedding of wood shavings and wet hay as roughage). Gelatin zymography was performed for qualitative and semi-quantitative evaluation of MMP-2 and MMP-9 in BALF supernatant, while fluorimetry was used to evaluate MMP-8 activity. Additionally, specific equine ELISA assays were used for quantitative assessment of MMP-2, MMP-9, TIMP-1 and TIMP-2.

resultsA significant reduction in the total and several single parameters of the clinical score were found after 10 days of inhalative therapy and EDR. The concentrations of MMP-2, MMP-9, TIMP-1 and TIMP-2 (ELISA) as well as their activities (MMP-2 and MMP-9 zymography and MMP-8 fluorimetry) were significantly decreased after therapy. Significant improvements in MMP-8/TIMP-1 and MMP-8/TIMP-2 ratios were also found, differences between other ratios before and after therapy were insignificant.

conclusionsMetalloproteinases and their inhibitors, in particular MMP-9 and TIMP-2, are valuable markers for clinical improvement in RAO.

Indexed as

DustAdministration, InhalationAirway ObstructionAnimalsBiomarkersFemaleGlucocorticoidsHorse DiseasesHorsesMaleMatrix Metalloproteinase InhibitorsMatrix MetalloproteinasesBiomarkersDustGlucocorticoidsMatrix Metalloproteinase InhibitorsMatrix MetalloproteinasesHorseInflammatory markerLungMetalloproteinasesRecurrent airway obstructionTissue inhibitors of metalloproteinases

Identifiers

PMID27938355
PMCPMC5148864
OpenAlexW2559986904

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.