ArticlePLoS pathogens2016
Gene Expression and Silencing Studies in Phytophthora infestans Reveal Infection-Specific Nutrient Transporters and a Role for the Nitrate Reductase Pathway in Plant Pathogenesis.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 75 citations in OpenAlex.
- Transcriptional programs controlling sporulation in Phytophthora respond to nutrition, humidity, and cryptochrome-mediated light signals.PLoS pathogens · 2026Article
- Host microenvironment in potato-Phytophthora infestans interaction revealed by single-cell spatiotemporal transcriptome.Nature plants · 2026Article
- Pathogenicity and virulence ofVirulence · 2025Review
- Oomycetes manipulate plant innate immunity through galacturonide oxidases.Nature communications · 2025Article
- Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion.Molecular plant pathology · 2025Article
- Host-induced gene silencing of the amino acid biosynthesis gene acetolactate synthase of Phytophthora infestans caused strong enhanced late blight resistance of potato in the field.Plant biotechnology journal · 2025Article
- Article
- RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Crossed wires: diatom phosphate sensing mechanisms coordinate nitrogen metabolism.Plant signaling & behavior · 2024Article
- Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.Molecular plant pathology · 2024Article
- Transcription factor binding specificities of the oomycete Phytophthora infestans reflect conserved and divergent evolutionary patterns and predict function.BMC genomics · 2024Article
- The Dynamic Interaction between Oil Palm andJournal of fungi (Basel, Switzerland) · 2024Article
- Dual transcriptomic analysis reveals early inducedFrontiers in plant science · 2024Article
- LAZARUS 1 functions as a positive regulator of plant immunity and systemic acquired resistance.Frontiers in plant science · 2024Article
- Iron transport pathways in the human malaria parasiteFrontiers in cellular and infection microbiology · 2024Article
- A comparison of transporter gene expression in three species of Peronospora plant pathogens during host infection.PloS one · 2023Article
- Strive or thrive: Trends inFrontiers in plant science · 2022Article
- A Cas12a-based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitor.Molecular plant pathology · 2021Article
- Review
- Uncovering the Role of Metabolism in Oomycete-Host Interactions Using Genome-Scale Metabolic Models.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To help learn how phytopathogens feed from their hosts, genes for nutrient transporters from the hemibiotrophic potato and tomato pest Phytophthora infestans were annotated. This identified 453 genes from 19 families. Comparisons with a necrotrophic oomycete, Pythium ultimum var. ultimum, and a hemibiotrophic fungus, Magnaporthe oryzae, revealed diversity in the size of some families although a similar fraction of genes encoded transporters. RNA-seq of infected potato tubers, tomato leaves, and several artificial media revealed that 56 and 207 transporters from P. infestans were significantly up- or down-regulated, respectively, during early infection timepoints of leaves or tubers versus media. About 17 were up-regulated >4-fold in both leaves and tubers compared to media and expressed primarily in the biotrophic stage. The transcription pattern of many genes was host-organ specific. For example, the mRNA level of a nitrate transporter (NRT) was about 100-fold higher during mid-infection in leaves, which are nitrate-rich, than in tubers and three types of artificial media, which are nitrate-poor. The NRT gene is physically linked with genes encoding nitrate reductase (NR) and nitrite reductase (NiR), which mobilize nitrate into ammonium and amino acids. All three genes were coregulated. For example, the three genes were expressed primarily at mid-stage infection timepoints in both potato and tomato leaves, but showed little expression in potato tubers. Transformants down-regulated for all three genes were generated by DNA-directed RNAi, with silencing spreading from the NR target to the flanking NRT and NiR genes. The silenced strains were nonpathogenic on leaves but colonized tubers. We propose that the nitrate assimilation genes play roles both in obtaining nitrogen for amino acid biosynthesis and protecting P. infestans from natural or fertilization-induced nitrate and nitrite toxicity.
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