ArticlePloS one2016
Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 122 citations in OpenAlex.
- In silico discovery of natural compounds from Vietnamese essential oils database with target binding to cancer-related proteins.Molecular diversity · 2026Article
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- Evaluation of Chemical Composition, Acaricidal, and Repellent Activities ofMolecules (Basel, Switzerland) · 2025Article
- De novo Transcriptomic analysis to unveil the deltamethrin induced resistance mechanisms in Callosobruchus chinensis (L.).Scientific reports · 2025Article
- Chemical Composition of Essential Oils and Their Potential Applications in Postharvest Storage of Cereal Grains.Molecules (Basel, Switzerland) · 2025Review
- Is the Biopesticide from Tea Tree Oil an Effective and Low-Risk Alternative to Chemical Pesticides? A Critical Review.Molecules (Basel, Switzerland) · 2024Review
- Target Enzymes ofInsects · 2024Article
- Antimicrobial and acaricide sanitizer tablets produced by wet granulation of spray-dried soap and clove oil-loaded microemulsion.PloS one · 2024Article
- Plant Essential Oils as Biopesticides: Applications, Mechanisms, Innovations, and Constraints.Plants (Basel, Switzerland) · 2023Review
- Fumigant activity and transcriptomic analysis of two plant essential oils against the tea green leafhopper,Frontiers in physiology · 2023Article
- Genome-Wide Identification of the Long Noncoding RNAs ofInsects · 2022Article
- Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean PestFrontiers in physiology · 2022Article
- Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole.Journal of insect science (Online) · 2022Article
- Study on the anti-biofilm mechanism of 1,8-cineole againstFrontiers in pharmacology · 2022Article
- Article
- Toxicological Stability ofMolecules (Basel, Switzerland) · 2021Article
- Evaluation of Aphicidal Effect of Essential Oils and Their Synergistic Effect againstMolecules (Basel, Switzerland) · 2021Article
- Stop the crop: Insights into the insecticidal mode of action of cinnamodial against mosquitoes.Pesticide biochemistry and physiology · 2021Article
- Article
- Insecticidal Activity ofFrontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cereal weevil, Sitophilus zeamais is one of the most destructive pests of stored cereals worldwide. Frequent use of fumigants for managing stored-product insects has led to the development of resistance in insects. Essential oils from aromatic plants including the tea oil plant, Melaleuca alternifolia may provide environmentally friendly alternatives to currently used pest control agents. However, little is known about molecular events involved in stored-product insects in response to plant essential oil fumigation.
resultsM. alternifolia essential oil was shown to possess the fumigant toxicity against S. zeamais. The constituent, terpinen-4-ol was the most effective compound for fumigant toxicity. M. alternifolia essential oil significantly inhibited the activity of three enzymes in S. zeamais, including two detoxifying enzymes, glutathione S-transferase (GST), and carboxylesterase (CarE), as well as a nerve conduction enzyme, acetylcholinesterase (AChE). Comparative transcriptome analysis of S. zeamais through RNA-Seq identified a total of 3,562 differentially expressed genes (DEGs), of which 2,836 and 726 were up-regulated and down-regulated in response to M. alternifolia essential oil fumigation, respectively. Based on gene ontology (GO) analysis, the majority of DEGs were involved in insecticide detoxification and mitochondrial function. Furthermore, an abundance of DEGs mapped into the metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were associated with respiration and metabolism of xenobiotics, including cytochrome P450s, CarEs, GSTs, and ATP-binding cassette transporters (ABC transporters). Some DEGs mapped into the proteasome and phagosome pathway were found to be significantly enriched. These results led us to propose a model of insecticide action that M. alternifolia essential oil likely directly affects the hydrogen carrier to block the electron flow and interfere energy synthesis in mitochondrial respiratory chain.
conclusionThis is the first study to perform a comparative transcriptome analysis of S. zeamais in response to M. alternifolia essential oil fumigation. Our results provide new insights into the insecticidal mechanism of M. alternifolia essential oil fumigation against S. zeamais and eventually contribute to the management of this important agricultural pest.
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