ArticlePloS one2016
Ex Vivo Lung Perfusion in the Rat: Detailed Procedure and Videos.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Research advances in consistency and variability inJournal of thoracic disease · 2026Review
- Comparative evaluation of PEGylated bovine hemoglobin as a perfusate in rat ex vivo lung perfusion.Respiratory research · 2026Article
- Comparative evaluation of single-artery cannulation with passive venous drainage versus traditional dual-cannula ex vivo lung perfusion in a rat model.Animal models and experimental medicine · 2026Article
- Effects of 3,3',5-triiodothyronine supplementation during ex vivo lung perfusion on ischemia/reperfusion injury in rat lungs.Frontiers in medicine · 2026Article
- An optimized perfusate for enhanced rat ex vivo lung perfusion and lung transplant models.Animal models and experimental medicine · 2025Article
- A proof-of-concept study in small and large animal models for coupling liver normothermic machine perfusion with mesenchymal stromal cell bioreactors.Nature communications · 2025Article
- Ex Vivo Organ Perfusion Systems for Disease Modeling and Therapeutic Applications in Small Animal Models.Journal of tissue engineering and regenerative medicine · 2025Review
- The establishment of anJournal of thoracic disease · 2024Article
- MG53 mitigates warm ischemic lung injury in a murine model of transplantation.The Journal of thoracic and cardiovascular surgery · 2024Article
- Twelve-hour normothermic liver perfusion in a rat model: characterization of the changes in the ex-situ bio-molecular phenotype and metabolism.Scientific reports · 2024Article
- Mitsugumin 53 mitigation of ischemia-reperfusion injury in a mouse model.The Journal of thoracic and cardiovascular surgery · 2024Article
- Low-VolumeTransplant international : official journal of the European Society for Organ Transplantation · 2024Article
- Design and Implementation of a Rat Ex Vivo Lung Perfusion Model.Journal of visualized experiments : JoVE · 2023Article
- Normothermic ex vivo lung perfusion outperforms conventional cold preservation in a deceased rat lung.Annals of translational medicine · 2022Article
- Ex Vivo Perfusion With Methylprednisolone Attenuates Brain Death-induced Lung Injury in Rats.Transplantation direct · 2021Article
- A translational rat model for ex vivo lung perfusion of pre-injured lungs after brain death.PloS one · 2021Article
- Human Red Blood Cells as Oxygen Carriers to Improve Ex-Situ Liver Perfusion in a Rat Model.Journal of clinical medicine · 2019Article
- Long agonal period deteriorates cardiac death donor lung function in a rat EVLP model.General thoracic and cardiovascular surgery · 2019Article
- Proteome Investigation of Rat Lungs subjected to Ex Vivo Perfusion (EVLP).Molecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ex vivo lung perfusion (EVLP) is a promising procedure for evaluation, reconditioning, and treatment of marginal lungs before transplantation. Small animal models can contribute to improve clinical development of this technique and represent a substantial platform for bio-molecular investigations. However, to accomplish this purpose, EVLP models must sustain a prolonged reperfusion without pharmacological interventions. Currently available protocols only partly satisfy this need. The aim of the present research was accomplishment and optimization of a reproducible model for a protracted rat EVLP in the absence of anti-inflammatory treatment. A 180 min, uninjured and untreated perfusion was achieved through a stepwise implementation of the protocol. Flow rate, temperature, and tidal volume were gradually increased during the initial reperfusion phase to reduce hemodynamic and oxidative stress. Low flow rate combined with open atrium and protective ventilation strategy were applied to prevent lung damage. The videos enclosed show management of the most critical technical steps. The stability and reproducibility of the present procedure were confirmed by lung function evaluation and edema assessment. The meticulous description of the protocol provided in this paper can enable other laboratories to reproduce it effortlessly, supporting research in the EVLP field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.