ArticlePloS one2016
Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1) Regulation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Harnessing the PpHog1 Regulatory Network to Engineer a UPR-Adaptive Komagataella phaffii Chassis for High-Copy Secretory Protein Production.Microbial biotechnology · 2026Article
- Flo5-1 and Nrg1 are involved in reversible pH-dependent flocculation in Komagataella phaffii.Applied microbiology and biotechnology · 2025Article
- Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions inJournal of fungi (Basel, Switzerland) · 2024Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- Improving AOX1 promoter efficiency by overexpression of Mit1 transcription factor.Molecular biology reports · 2022Article
- Expanding the promoter toolbox for metabolic engineering of methylotrophic yeasts.Applied microbiology and biotechnology · 2022Review
- Article
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Coordinate regulation of methanol utilization pathway genes ofFrontiers in microbiology · 2022Article
- Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the YeastMicroorganisms · 2021Article
- Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.Current genetics · 2021Article
- Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris.AMB Express · 2020Article
- Transcriptomic Analysis ofFrontiers in microbiology · 2020Article
- Modulation of acetate utilization inBiotechnology for biofuels · 2019Article
- Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As one of the most commonly used eukaryotic recombinant protein expression systems, P. pastoris relies heavily on the AOX1 promoter (PAOX1), which is strongly induced by methanol but strictly repressed by glycerol and glucose. However, the complicated signaling pathways involved in PAOX1 regulation when supplemented with different carbon sources are poorly understood. Here we constructed a kinase deletion library in P. pastoris and identified 27 mutants which showed peculiar phenotypes in cell growth or PAOX1 regulation. We analyzed both annotations and possible functions of these 27 targets, and then focused on the MAP kinase Hog1. In order to locate its potential downstream components, we performed the phosphoproteome analysis on glycerol cultured WT and Δhog1 strains and identified 157 differentially phosphorylated proteins. Our results identified important kinases involved in P. pastoris cell growth and PAOX1 regulation, which could serve as valuable targets for further mechanistic studies.
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