Evidence map›Paper›PMID 27935983›Full record

ArticlePloS one2016

Involvement of Pancreatic Stellate Cells in Regeneration of Remnant Pancreas after Partial Pancreatectomy.

Shigenori Ota, Miyuki Nishimura, Yuya Murakami, Naoko Kubo Birukawa, Akihiro Yoneda, Hiroki Nishita, Ryosuke Fujita, Yasushi Sato, Kenjiro Minomi, Keiko Kajiwara and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 3 institutions in 1 country.

Shigenori OtaDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Miyuki NishimuraDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Yuya MurakamiDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Naoko Kubo BirukawaDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Akihiro YonedaDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Hiroki NishitaDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Ryosuke FujitaDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Yasushi SatoDepartment of Medical Oncology and Hematology Sapporo Medical University, Sapporo, Japan.
Kenjiro MinomiDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Keiko KajiwaraDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Miyono MiyazakiDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Maki UchiumiDepartment of Molecular Therapeutics, Center for Food & Medical Innovation Hokkaido University, Japan.
Shintaro MikuniDepartment of Molecular Therapeutics, Center for Food & Medical Innovation Hokkaido University, Japan.
Yasuaki TamuraDepartment of Molecular Therapeutics, Center for Food & Medical Innovation Hokkaido University, Japan.
Toru MizuguchiDepartment of Surgery, Surgical Oncology & Science, Sapporo Medical University, Sapporo, Japan.
Masafumi ImamuraDepartment of Surgery, Surgical Oncology & Science, Sapporo Medical University, Sapporo, Japan.
Makoto MeguroDepartment of Surgery, Surgical Oncology & Science, Sapporo Medical University, Sapporo, Japan.
Yasutoshi KimuraDepartment of Surgery, Surgical Oncology & Science, Sapporo Medical University, Sapporo, Japan.
Koichi HirataDepartment of Surgery, Surgical Oncology & Science, Sapporo Medical University, Sapporo, Japan.
Yoshiro NiitsuDepartment of Molecular Target Exploration, Sapporo Medical University, Sapporo, Japan.
Sapporo Medical University · JPHokkaido University · JPNitto (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesMechanism of regeneration of remnant pancreas after partial pancreatectomy (PX) is still unknown. In this study, effect of siRNA against the collagen specific chaperone, HSP47, which inhibits collagen secretion from activated pancreas stellate cells (aPSCs), and induces their apoptosis, on regeneration of remnant pancreas was determined.

methodsPancreatectomy was performed according to established methods. Proliferation of cells was assessed by BrdU incorporation. Immunostaining of HSP47 was employed to identify PSCs. Progenitor cells were identified by SOX9 staining. Acinar cells were immunostained for amylase. Co-culture of acinar cells with aPSCs were carried out in a double chamber with a cell culture insert. siRNA HSP47 encapsulated in vitamin A-coupled liposome (VA-lip siRNA HSP47) was delivered to aPSCs by iv injection.

resultsIn remnant pancreas of 90% PX rat, new areas of foci were located separately from duodenal areas with normal pancreatic features. After PX, BrdU uptake of acinar cells and islet cells significantly increased, but was suppressed by treatment with VA-lip siRNA HSP47. BrdU uptake by acinar cells was augmented by co-culturing with aPSCs and the augmentation was nullified by siRNA HSP47. BrdU uptake by progenitor cells in foci area was slightly enhanced by the same treatment. New area which exhibited intermediate features between those of duodenal and area of foci, emerged after the treatment.

conclusionaPSCs play a crucial role in regeneration of remnant pancreas, proliferation of acinar and islet cells after PX through the activity of secreted collagen. Characterization of new area emerged by siRNA HSP47 treatment as to its origin is a future task.

Indexed as

Acinar CellsAnimalsBiomarkersCell ProliferationCoculture TechniquesGene ExpressionHSP47 Heat-Shock ProteinsIslets of LangerhansLiposomesMalePancreasPancreatectomyPancreatic Stellate CellsRatsRats, Sprague-DawleyRegenerationBiomarkersHSP47 Heat-Shock ProteinsLiposomesRNA, Small InterferingSerpinh1 protein, ratSOX9 Transcription FactorVitamin A

Identifiers

PMID27935983
PMCPMC5147817
OpenAlexW2560508005

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.