ArticlePloS one2016
A Cost-Effective Method to Assemble Biomimetic 3D Cell Culture Platforms.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The Application of ImageJ Software for Roughness Analysis of Dental Implants.Journal of imaging informatics in medicine · 2025Article
- Human amniotic membrane scaffold enhances adipose mesenchymal stromal cell mitochondrial bioenergetics promoting their regenerative capacities.Molecular and cellular biochemistry · 2025Article
- Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stromal/Stem Cell Seeding.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of the Human Amniotic Membrane as an Adjuvant Therapy for the Treatment of Hepatocellular Carcinoma.Advances in experimental medicine and biology · 2024Review
- Targeting the epigenome of cancer stem cells in pediatric nervous system tumors.Molecular and cellular biochemistry · 2023Review
- Efficient Generation of Chondrocytes From Bone Marrow-Derived Mesenchymal Stem Cells in a 3D Culture System: Protocol for a Practical Model for Assessing Anti-Inflammatory Therapies.JMIR research protocols · 2023Article
- Fabrication of Decellularized Amnion and Chorion Scaffolds to Develop Bioengineered Cell-Laden Constructs.Cellular and molecular bioengineering · 2022Article
- Applications of the amniotic membrane in tissue engineering and regeneration: the hundred-year challenge.Stem cell research & therapy · 2022Review
- Three-Dimensional Culture ofInsects · 2021Article
- Combined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model.Calcified tissue international · 2021Article
- Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing.Bioengineering (Basel, Switzerland) · 2020Review
- Efficiency of Differently Processed Membranes Based on Cellulose as Cationic Dye Adsorbents.Nanomaterials (Basel, Switzerland) · 2020Article
- Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model.Journal of cellular and molecular medicine · 2019Article
- Development of decellularized amniotic membrane as a bioscaffold for bone marrow-derived mesenchymal stem cells: ultrastructural study.Journal of molecular histology · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
methodsWe utilized the hAM to provide the biological and the three dimensional (3D) topographic components of the prototype. The 3D nano-roughness of the hAM was characterized using surface electron microscopy and surface image analysis (ImageJ and SurfaceJ). We developed additional macro-scale and micro-scale versions of the platform which provided additional shear stress factors to simulate the fluid dynamics of the in vivo extracellular fluids.
resultsThree models of varying complexities of the prototype were assembled. A well-defined 3D surface modulation of the hAM in comparable to commercial 3D biomaterial culture substrates was achieved without complex fabrication and with significantly lower cost. Performance of the prototype was demonstrated through culture of primary human umbilical cord mononuclear blood cells (MNCs), human bone marrow mesenchymal stem cell line (hBMSC), and human breast cancer tissue.
conclusionThis study presents methods of assembling an integrated, flexible and low cost biomimetic cell culture platform for diverse cell culture applications.
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