ArticlePLoS genetics2016
Alternative Splicing within and between Drosophila Species, Sexes, Tissues, and Developmental Stages.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Full-Length Transcriptome Comparison of Male and Female Gonads inBiomolecules · 2026Article
- A framework for identifying transcript orthologs: the evolution of sex bias in alternative transcript structure in Drosophila.bioRxiv : the preprint server for biology · 2026Article
- CRESCENT, a comprehensive RNA-Seq expression, splicing, and coding/non-coding element network tool.BMC bioinformatics · 2026Article
- Butterfly brains change in morphology and in gene splicing patterns after brief pheromone exposure.BMC biology · 2026Article
- A Comparative Analysis of Transcriptome-Wide Differential Gene Expression and Alternative Polyadenylation in the Ovaries of Meat Ducks and Laying Ducks.Animals : an open access journal from MDPI · 2026Article
- Review
- Article
- Orthologous marker groups reveal broad cell identity conservation across plant single-cell transcriptomes.Nature communications · 2025Article
- Identification of Splicing Variation Associated with Parental Behavior in the Burying Beetles (Journal of genomics · 2025Article
- Full-Length Transcriptome Profile ofInternational journal of molecular sciences · 2024Article
- Diverse somatic Transformer and sex chromosome karyotype pathways regulate gene expression in Drosophila gonad development.bioRxiv : the preprint server for biology · 2024Article
- Article
- Sex Differences in Cochlear Transcriptomes in Horseshoe Bats.Animals : an open access journal from MDPI · 2024Article
- Nucleotide-level distance metrics to quantify alternative splicing implemented in TranD.Nucleic acids research · 2024Article
- Quantifying transcriptome diversity: a review.Briefings in functional genomics · 2024Review
- Identification of antennal alternative splicing by combining genome and full-length transcriptome analysis inFrontiers in physiology · 2024Article
- Article
- Estimating transcriptome complexities across eukaryotes.BMC genomics · 2023Article
- Two Forms of Sexual Dimorphism in Gene Expression in Drosophila melanogaster: Their Coincidence and Evolutionary Genetics.Molecular biology and evolution · 2023Article
- A Global Survey of the Full-Length Transcriptome ofInternational journal of molecular sciences · 2023Article
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4 authors.
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Abstract
Alternative pre-mRNA splicing ("AS") greatly expands proteome diversity, but little is known about the evolutionary landscape of AS in Drosophila and how it differs between embryonic and adult stages or males and females. Here we study the transcriptomes from several tissues and developmental stages in males and females from four species across the Drosophila genus. We find that 20-37% of multi-exon genes are alternatively spliced. While males generally express a larger number of genes, AS is more prevalent in females, suggesting that the sexes adopt different expression strategies for their specialized function. While the number of total genes expressed increases during early embryonic development, the proportion of expressed genes that are alternatively spliced is highest in the very early embryo, before the onset of zygotic transcription. This indicates that females deposit a diversity of isoforms into the egg, consistent with abundant AS found in ovary. Cluster analysis by gene expression ("GE") levels shows mostly stage-specific clustering in embryonic samples, and tissue-specific clustering in adult tissues. Clustering embryonic stages and adult tissues based on AS profiles results in stronger species-specific clustering, suggesting that diversification of splicing contributes to lineage-specific evolution in Drosophila. Most sex-biased AS found in flies is due to AS in gonads, with little sex-specific splicing in somatic tissues.
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