Evidence map›Paper›PMID 27935613›Full record

ArticleChemical communications (Cambridge, England)2016

Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells.

Pedro M S D Cal, Florian Sieglitz, Fábio M F Santos, Cátia Parente Carvalho, Ana Guerreiro, Jean B Bertoldo, Uwe Pischel, Pedro M P Gois, Gonçalo J L Bernardes

Open access · hybridAbstract read
In one paragraph

Article in Chemical communications (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 31 citations in OpenAlex.

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  5. Platform for OrthogonalJournal of the American Chemical Society · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 5 countries.

Pedro M S D CalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt and Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
Florian SieglitzInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt.
Fábio M F SantosResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal. pedrogois@ff.ul.pt.
Cátia Parente CarvalhoCIQSO - Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, 21071 Huelva, Spain.
Ana GuerreiroInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt.
Jean B BertoldoDepartment of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
Uwe PischelCIQSO - Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, 21071 Huelva, Spain.
Pedro M P GoisResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal. pedrogois@ff.ul.pt.
Gonçalo J L BernardesInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt and Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.ORCID 0000-0001-6594-8917
University of Lisbon · PTUniversidad de Huelva · ESUniversity of Cambridge · GB

Funding

European Research Council 676832
6 · The paper itself

Abstract

Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.

Indexed as

ApoptosisAnnexin A5Boronic AcidsFluorescent DyesHeLa CellsHumansHydrazonesMicroscopy, ConfocalModels, MolecularMolecular ConformationAnnexin A5Boronic AcidsFluorescent DyesHydrazones

Identifiers

PMID27935613
PMCPMC6485347
OpenAlexW2558525175

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.