ArticleThe ISME journal2017
Single-cell mass spectrometry reveals the importance of genetic diversity and plasticity for phenotypic variation in nitrogen-limited Chlamydomonas.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 29 citations in OpenAlex.
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- Mass Spectrometry-based Single-Cell Lipidomics: Advancements, Challenges, and the Path Forward.Trends in analytical chemistry : TRAC · 2023Article
- RespectM revealed metabolic heterogeneity powers deep learning for reshaping the DBTL cycle.iScience · 2023Article
- FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins in cells of different lineages.Journal of lipid research · 2023Article
- Mass spectrometry imaging to explore molecular heterogeneity in cell culture.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Microbial metabolic noise.WIREs mechanisms of disease · 2021Review
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Identification to species level of live single microalgal cells from plankton samples with matrix-free laser/desorption ionization mass spectrometry.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Single-Cell Technologies to Understand the Mechanisms of Cellular Adaptation in Chemostats.Frontiers in bioengineering and biotechnology · 2020Article
- The emergence of metabolic heterogeneity and diverse growth responses in isogenic bacterial cells.The ISME journal · 2018Article
- Article
- Individual-level trait diversity predicts phytoplankton community properties better than species richness or evenness.The ISME journal · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Phenotypic variation is vital for microbial populations to survive environmental perturbations. Both genetic and non-genetic factors contribute to an organism's phenotypic variation and therefore its fitness. To investigate the correlation between genetic diversity and phenotypic variation, we applied our recently developed mass spectrometry method that allows for the simultaneous measurement of more than 25 different lipids and pigments with high throughput in the unicellular microalga Chlamydomonas reinhardtii. We monitored the impact of nitrogen limitation on a genetically diverse wild-type strain CC-1690 and two isoclonal isolates from CC-1690 named ANC3 and ANC5. Measuring molecular composition of thousands of single cells at different time points of the experiment allowed us to capture a dynamic picture of the phenotypic composition and adaptation of the populations over time. Although the genetically diverse population maintained phenotypic variation over the whole time course of the experiment, the isoclonal cultures showed higher synchronicity in their phenotypic response. Furthermore, the genetically diverse population showed equal or greater phenotypic variation over the whole time range in multidimensional trait space compared with isoclonal populations. However, along individual trait axes non-genetic variance was higher in isoclonal populations.
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