ArticleAnalytical chemistry2017
Precast Gelatin-Based Molds for Tissue Embedding Compatible with Mass Spectrometry Imaging.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- The evolution of DESI-MSI: A comprehensive look at preprocessing and quantification strategies for metabolomics.Journal of food and drug analysis · 2026Review
- Method development for correlating lipid molecular information with anatomy in C. elegans.Scientific reports · 2025Article
- Quality Control Standards for Batch Effect Evaluation and Correction in Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Reproducible Sectioning and Sample Preparation for Mass Spectrometry Imaging of Cancer Metabolism in Frozen Lung Tissue.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Amorphous Titanium Dioxide Nanoparticles and Their Unexpected Fragmentation in MALDI-TOF/MS.ACS omega · 2024Article
- MALDI-TOF imaging analysis of benzalkonium chloride penetration in ex vivo human skin.PloS one · 2024Article
- Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.Scientific reports · 2023Article
- Chemical QuantArray: A Quantitative Tool for Mass Spectrometry Imaging.Analytical chemistry · 2023Article
- Tissue Microarray Lipidomic Imaging Mass Spectrometry Method: Application to the Study of Alcohol-Related White Matter Neurodegeneration.Applied biosciences · 2023Article
- Review
- Mass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids.Frontiers in molecular biosciences · 2023Article
- Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mass spectrometry imaging: new eyes on natural products for drug research and development.Acta pharmacologica Sinica · 2022Review
- [Performance Comparison of Two Cryosection Embedding Agents Used for Desorption Electrospray Ionization Mass Spectrometry Imaging].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Article
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- Mapping the Chemistry of Hair Strands by Mass Spectrometry Imaging-A Review.Molecules (Basel, Switzerland) · 2021Review
- Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging.Toxins · 2021Article
- Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.STAR protocols · 2021Article
- Removal of optimal cutting temperature (O.C.T.) compound from embedded tissue for MALDI imaging of lipids.Analytical and bioanalytical chemistry · 2021Article
- Novel technical developments in mass spectrometry imaging in 2020: A mini review.Analytical science advances · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Preparation of tissue for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) generally involves embedding the tissue followed by freezing and cryosectioning, usually between 5 and 25 μm thick, depending on the tissue type and the analyte(s) of interest. The brain is approximately 60% fat; it therefore lacks rigidity and poses structural preservation challenges during sample preparation. Histological sample preparation procedures are generally transferable to MALDI-MSI; however, there are various limitations. Optimal cutting temperature compound (OCT) is commonly used to embed and mount fixed tissue onto the chuck inside the cryostat during cryosectioning. However, OCT contains potential interferences that are detrimental to MALDI-MSI, while fixation is undesirable for the analysis of some analytes either due to extraction or chemical modification (i.e., polar metabolites). Therefore, a method for both fixed and fresh tissue compatible with MALDI-MSI and histology is desirable to increase the breadth of analyte(s), maintain the topographies of the brain, and provide rigidity to the fragile tissue while eliminating background interference. The method we introduce uses precast gelatin-based molds in which a whole mouse brain is embedded, flash frozen, and cryosectioned in preparation for mass spectrometry imaging (MSI).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.