ArticleCold Spring Harbor protocols2016
Gene-Centered Yeast One-Hybrid Assays.
Article in Cold Spring Harbor protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- IbMYB1-4 positively regulates purple stem and influences leaf color via dosage effect of gene expression in sweetpotato (Ipomoea batatas (L.) Lam.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Candidate promoter-associated factors ofFrontiers in plant science · 2026Article
- From binding to networks: methods for identifying transcription factor targets in plant systems.Frontiers in plant science · 2026Review
- Review
- A large-scale cancer-specific protein-DNA interaction network.bioRxiv : the preprint server for biology · 2024Article
- Construction of Yeast One-Hybrid Library ofJournal of fungi (Basel, Switzerland) · 2023Article
- Deciphering Novel Transcriptional Regulators of Soybean Hypocotyl Elongation Based on Gene Co-expression Network Analysis.Frontiers in plant science · 2022Article
- Multiple Antitumor Molecular Mechanisms Are Activated by a Fully Synthetic and Stabilized Pharmaceutical Product Delivering the Active Compound Sulforaphane (SFX-01) in Preclinical Model of Human Glioblastoma.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Identification of genomic enhancers through spatial integration of single-cell transcriptomics and epigenomics.Molecular systems biology · 2020Article
- Discovering human transcription factor physical interactions with genetic variants, novel DNA motifs, and repetitive elements using enhanced yeast one-hybrid assays.Genome research · 2019Article
- Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences.Journal of visualized experiments : JoVE · 2019Article
- Global landscape of mouse and human cytokine transcriptional regulation.Nucleic acids research · 2018Article
- Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.Zebrafish · 2018Article
- Protein Interactomics by Two-Hybrid Methods.Methods in molecular biology (Clifton, N.J.) · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An important question when studying gene regulation is which transcription factors (TFs) interact with which cis-regulatory elements, such as promoters and enhancers. Addressing this issue in complex multicellular organisms is challenging as several hundreds of TFs and thousands of regulatory elements must be considered in the context of different tissues and physiological conditions. Yeast one-hybrid (Y1H) assays provide a powerful "gene-centered" method to identify the TFs that can bind a DNA sequence of interest. In this introduction, we describe the basic principles of the Y1H assay and its advantages and disadvantages and briefly discuss how it is complementary to "TF-centered" methods that identify protein-DNA interactions for a known protein of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.