ArticleJournal of the American Chemical Society2016
Development of Correlative Cryo-soft X-ray Tomography and Stochastic Reconstruction Microscopy. A Study of Cholesterol Crystal Early Formation in Cells.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- 3D cryoimaging of cell-mediated cholesterol crystal clearance in human atherosclerotic lesions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Direct observation of cholesterol monohydrate crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid Droplet Surface Promotes 3D Morphological Evolution of Non-Rhomboidal Cholesterol Crystals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography.Journal of structural biology: X · 2024Article
- Selective regulation of macrophage lipid metabolism via nanomaterials' surface chemistry.Nature communications · 2024Article
- Mitigating Vascular Inflammation by Mimicking AIBP Mechanisms: A New Therapeutic End for Atherosclerotic Cardiovascular Disease.International journal of molecular sciences · 2024Review
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- β-Cyclodextrin-based nanoassemblies for the treatment of atherosclerosis.Regenerative biomaterials · 2024Review
- Biomimetic nanoparticles to enhance the reverse cholesterol transport for selectively inhibiting development into foam cell in atherosclerosis.Journal of nanobiotechnology · 2023Article
- On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography.Micromachines · 2023Article
- Advanced Light Source Analytical Techniques for Exploring the Biological Behavior and Fate of Nanomedicines.ACS central science · 2022Review
- Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences.Biochemical Society transactions · 2022Article
- Pathways and Mechanisms of Cellular Cholesterol Efflux-Insight From Imaging.Frontiers in cell and developmental biology · 2022Review
- Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.Nature protocols · 2021Article
- Article
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Correlative cryo-soft X-ray tomography of cells.Biophysical reviews · 2019Article
- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.Methods in molecular biology (Clifton, N.J.) · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
We have developed a high resolution correlative method involving cryo-soft X-ray tomography (cryo-SXT) and stochastic optical reconstruction microscopy (STORM), which provides information in three dimensions on large cellular volumes at 70 nm resolution. Cryo-SXT morphologically identified and localized aggregations of carbon-rich materials. STORM identified specific markers on the desired epitopes, enabling colocalization between the identified objects, in this case cholesterol crystals, and the cellular environment. The samples were studied under ambient and cryogenic conditions without dehydration or heavy metal staining. The early events of cholesterol crystal development were investigated in relation to atherosclerosis, using as model macrophage cell cultures enriched with LDL particles. Atherosclerotic plaques build up in arteries in a slow process involving cholesterol crystal accumulation. Cholesterol crystal deposition is a crucial stage in the pathological cascade. Our results show that cholesterol crystals can be identified and imaged at a very early stage on the cell plasma membrane and in intracellular locations. This technique can in principle be applied to other biological samples where specific molecular identification is required in conjunction with high resolution 3D-imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.