ArticleCytometry. Part B, Clinical cytometry2017
Mass Cytometry Analytical Approaches Reveal Cytokine-Induced Changes in Natural Killer Cells.
Article in Cytometry. Part B, Clinical cytometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- CD56-negative NK cells: Frequency in peripheral blood, expansion during HIV-1 infection, functional capacity, and KIR expression.Frontiers in immunology · 2022Pooled it
- Activatory Receptor NKp30 Predicts NK Cell Activation During Controlled Human Malaria Infection.Frontiers in immunology · 2019Trial
- Transcriptional Profile Change of NK-92 Cells in Presence of Cytokines, TGFβ Signaling Pathway Inhibitor and CDK7/12/13 Kinase Inhibitor.International journal of molecular sciences · 2026Article
- NKp30 and NKG2D contribute to natural killer cell-mediated recognition of HIV-infected cells.iScience · 2025Article
- Enhanced expression of natural cytotoxicity receptors on cytokine-induced memory-like natural killer cells correlates with effector function.Frontiers in immunology · 2023Article
- Cytokine-Induced Memory-Like NK Cells: From the Basics to Clinical Applications.Frontiers in immunology · 2022Review
- Contribution of natural killer cells in innate immunity against colorectal cancer.Frontiers in oncology · 2022Review
- Gut Bacteria Induce Granzyme B Expression in Human Colonic ILC3s In Vitro in an IL-15-Dependent Manner.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Metabolic changes of Interleukin-12/15/18-stimulated human NK cells.Scientific reports · 2021Article
- Natural Killer (NK) Cell Expression of CD2 as a Predictor of Serial Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC).Antibodies (Basel, Switzerland) · 2020Article
- Progress and applications of mass cytometry in sketching immune landscapes.Clinical and translational medicine · 2020Review
- Charge-altering releasable transporters enable phenotypic manipulation of natural killer cells for cancer immunotherapy.Blood advances · 2020Article
- Treated HIV Infection Alters Phenotype but Not HIV-Specific Function of Peripheral Blood Natural Killer Cells.Frontiers in immunology · 2020Article
- Characterization of the Impact of Daclizumab Beta on Circulating Natural Killer Cells by Mass Cytometry.Frontiers in immunology · 2020Article
- The Evolutionary Arms Race between Virus and NK Cells: Diversity Enables Population-Level Virus Control.Viruses · 2019Review
- Differential Induction of IFN-α and Modulation of CD112 and CD54 Expression Govern the Magnitude of NK Cell IFN-γ Response to Influenza A Viruses.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Diversification of human NK cells: Lessons from deep profiling.Journal of leukocyte biology · 2018Review
- Implication of Interleukin-12/15/18 and Ruxolitinib in the Phenotype, Proliferation, and Polyfunctionality of Human Cytokine-Preactivated Natural Killer Cells.Frontiers in immunology · 2018Article
- BPTF inhibits NK cell activity and the abundance of natural cytotoxicity receptor co-ligands.Oncotarget · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundNatural killer (NK) cells have antiviral and antitumor activity that could be harnessed for the treatment of infections and malignancies. To maintain cell viability and enhance antiviral and antitumor effects, NK cells are frequently treated with cytokines. Here they performed an extensive assessment of the effects of cytokines on the phenotype and function of human NK cells.
methodsThey used cytometry by time-of-flight (CyTOF) to evaluate NK cell repertoire changes after stimulation with interleukin (IL)-2, IL-15 or a combination of IL-12/IL-15/IL-18. To analyze the high dimensional CyTOF data, they used several statistical and visualization tools, including viSNE (Visualization of t-Distributed Stochastic Neighbor Embedding), Citrus (Cluster identification, characterization, and regression), correspondence analysis, and the Friedman-Rafsky test.
resultsAll three treatments (IL-2, IL-15, and IL-12/IL-15/IL-18) increase expression of CD56 and CD69. The effects of treatment with IL-2 and IL-15 are nearly indistinguishable and characterized principally by increased expression of surface markers including CD56, NKp30, NKp44, and increased expression of functional markers, such as perforin, granzyme B, and MIP-1β. The combination of IL-12/IL-15/IL-18 induces a profound shift in the repertoire structure, decreasing expression of CD16, CD57, CD8, NKp30, NKp46, and NKG2D, and dramatically increasing expression of IFN-γ.
conclusionsCyTOF provides insights into the effects of cytokines on the phenotype and function of NK cells, which could inform future research efforts and approaches to NK cell immunotherapy. There are several analytical approaches to CyTOF data, and the appropriate method should be carefully selected based on which aspect of the dataset is being explored. © 2016 International Clinical Cytometry Society.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.