Evidence map›Paper›PMID 27933493›Full record

ArticleFood and environmental virology2017

Norovirus GII.Pe Genotype: Tracking a Foodborne Outbreak on a Cruise Ship Through Molecular Epidemiology, Brazil, 2014.

Simone Guadagnucci Morillo, Adriana Luchs, Audrey Cilli, Cibele Daniel Ribeiro, Rita de Cássia Compagnoli Carmona, Maria do Carmo Sampaio Tavares Timenetsky

Abstract read
PubMed Publisher
In one paragraph

Article in Food and environmental virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Human norovirus in Brazil: an update of reports in different settings.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Review
  4. Article
  5. Evaluation of skimmed milk flocculation method for virus recovery from tomatoes.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2018
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Simone Guadagnucci MorilloEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil. sgmorillo@gmail.com.
Adriana LuchsEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil. driluchs@gmail.com.
Audrey CilliEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil.
Cibele Daniel RibeiroEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil.
Rita de Cássia Compagnoli CarmonaEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil.
Maria do Carmo Sampaio Tavares TimenetskyEnteric Diseases Laboratory, Virology Center, Adolfo Lutz Institute, Avenida Dr Arnaldo, No 355, São Paulo, SP, CEP 01246-902, Brazil. timenetsky.m@gmail.com.
Instituto Adolfo Lutz · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0-98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV.

Indexed as

AdolescentAdultAgedAged, 80 and overBrazilCaliciviridae InfectionsChildChild, PreschoolFemaleFoodborne DiseasesFood ContaminationGastroenteritisGenotypeHumansMaleMiddle AgedCruise ship outbreakFoodborne gastroenteritis outbreakNaturally contaminated food detectionNorovirus

Identifiers

PMID27933493
OpenAlexW2559903133

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.