Evidence map›Paper›PMID 27933102›Full record

ArticleBiotechnology for biofuels2016

A polysaccharide utilization locus from

Johan Larsbrink, Yongtao Zhu, Sampada S Kharade, Kurt J Kwiatkowski, Vincent G H Eijsink, Nicole M Koropatkin, Mark J McBride, Phillip B Pope

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 97 citations in OpenAlex.

  1. Article
  2. A system for transferring large genetic loci inApplied and environmental microbiology · 2026
    Article
  3. Review
  4. Co-zorbs: Motile, multispecies biofilms aid transport of diverse bacterial species.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Article
  7. Effects of Toxic and Non-ToxicMicroorganisms · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. The Utilization byFoods (Basel, Switzerland) · 2024
    Article
  13. mBio · 2024
    Article
  14. Article
  15. Differential gliding motility responses ofCurrent research in microbial sciences · 2024
    Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Johan LarsbrinkDepartment of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway ; Wallenberg Wood Science Center, Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.ORCID 0000-0001-8386-2914
Yongtao ZhuDepartment of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201 USA.
Sampada S KharadeDepartment of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201 USA.
Kurt J KwiatkowskiDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 USA.
Vincent G H EijsinkDepartment of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway.
Nicole M KoropatkinDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 USA.
Mark J McBrideDepartment of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201 USA.
Phillip B PopeDepartment of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway.
Norwegian University of Life Sciences · NOUniversity of Wisconsin–Milwaukee · USUniversity of Michigan · US

Funding

University of Michigan Center for Gastrointestinal ResearchP30DK034933 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI OWYANG, CHUNG · 1986 to 2021
$22.5M
NIDDK NIH HHS P30 DK034933
6 · The paper itself

Abstract

backgroundChitin is the second most abundant polysaccharide on earth and as such a great target for bioconversion applications. The phylum Bacteroidetes is one of nature's most ubiquitous bacterial lineages and is essential in the global carbon cycle with many members being highly efficient degraders of complex carbohydrates. However, despite their specialist reputation in carbohydrate conversion, mechanisms for degrading recalcitrant crystalline polysaccharides such as chitin and cellulose are hitherto unknown.

resultsHere we describe a complete functional analysis of a novel polysaccharide utilization locus (PUL) in the soil Bacteroidete

conclusionsBy combining reverse genetics to map essential PUL genes, structural studies on outer membrane chitin-binding proteins, and enzymology, we provide insight into the mechanisms employed by Bacteroidetes to degrade recalcitrant polysaccharides and introduce a new saccharolytic mechanism used by the phylum Bacteroidetes. The presented discovery and analysis of the ChiUL will greatly benefit future enzyme discovery efforts as well as studies regarding enzymatic intramolecular synergism.

Indexed as

BacteroidetesChitinPolysaccharide utilization locusRecalcitrant polysaccharides

Identifiers

PMID27933102
PMCPMC5127042
OpenAlexW2557698447

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.