ArticleBiodiversity data journal2016
Metabarcoding of marine nematodes - evaluation of similarity scores used in alignment-based taxonomy assignment approach.
Article in Biodiversity data journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Article
- Comparison of morphological, DNA barcoding, and metabarcoding characterizations of freshwater nematode communities.Ecology and evolution · 2020Article
- Improved phylogenomic sampling of free-living nematodes enhances resolution of higher-level nematode phylogeny.BMC evolutionary biology · 2019Article
- Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes.Royal Society open science · 2017Article
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Authors and funding
1 author.
Funding
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Abstract
backgroundThe diversity of organisms is being commonly accessed using metabarcoding of environmental samples. Reliable identification of barcodes is one of the critical steps in the process and several taxonomy assignment methods were proposed to accomplish this task, including alignment-based approach that uses Basic Local Alignment Search Tool (BLAST) algorithm. This publication evaluates the variability of 5' end of 18S rRNA barcoding region as expressed by similarity scores (alignment score and identity score) produced by BLAST, and its impact on barcode identification to family-level taxonomic categories. NEW INFORMATION: In alignment-based taxonomy assignment approach, reliable identification of anonymous OTUs to supraspecific taxa depends on the correct application of similarity thresholds. Since various taxa show different level of genetic variation, practical application of alignment-based approach requires the determination and use of taxon-specific similarity thresholds.
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