Evidence map›Paper›PMID 27931311›Full record

ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2016

Cryopreservation of Cynomolgus Macaque (Macaca fascicularis) Sperm by Using a Commercial Egg-YolkFree Freezing Medium.

Yaping Yan, Lei Ao, Hong Wang, Yanchao Duan, Shaohui Chang, Bingbing Chen, Dalong Zhi, Sujuan Li, Yuyu Niu, Weizhi Ji and 1 more

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Cryopreservation of Mauritian Cynomolgus Macaque (Journal of the American Association for Laboratory Animal Science : JAALAS · 2020
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yaping YanYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Lei AoKunming SinoUK Angel Women's and Children's Hospital, Kunming, Yunnan, China.
Hong WangYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Yanchao DuanYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Shaohui ChangYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Bingbing ChenYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Dalong ZhiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Sujuan LiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Yuyu NiuYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Weizhi JiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Wei SiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China;, Email: siwei76@hotmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional TRISegg yolk (TEY) freezing medium for the cryopreservation of NHP sperm has the risk of contamination due to widespread zoonotic diseases. This study was aimed at determining the optimal glycerol concentration, freezing rate, and holding time in liquid N2 vapor for the cryopreservation of cynomolgus macaque sperm by using a commercial egg-yolkfree freezing medium (SC medium) designed for human sperm cryopreservation. Sperm motility and acrosomal integrity after freezing were assessed. Sperm in SC medium (dilution ratio, 3:1) frozen at cooling rates of 67 and 183C/min in liquid N2 vapor showed higher post-thaw motility than did samples frozen at 435C/min. At the cooling rate of 183C/min and dilution in SC medium at a 3:1 ratio, post-thaw motility was higher after a holding time of 10 min than after 30 min (recommended by the manufacturer). In addition, post-thaw motility of sperm frozen in SC medium was higher with dilution ratios of 3:1, 4.5:1, and 6:1 compared with 9:1, 10.5:1, and 12:1, and the sample diluted 12:1 showed the lowest percentage of thawed sperm with intact acrosomes. Sperm showed higher post-thaw motility after freezing in TEY than in SC medium; acrosomal integrity did not differ between the 2 media. Our results indicated that cynomolgus macaque sperm can be cryopreserved successfully by using a commercial egg-yolkfree freezing medium, which provides an option for genetic preservation with decreased zoonotic risk in this important NHP species.

Indexed as

Macaca fascicularisAnimalsCryopreservationCryoprotective AgentsEggsHumansMaleSemen PreservationSpermatozoaSperm MotilityCryoprotective Agents

Identifiers

PMID27931311
PMCPMC5113874

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.