ArticleStem cell research & therapy2016
Extracellular matrix components and culture regimen selectively regulate cartilage formation by self-assembling human mesenchymal stem cells in vitro and in vivo.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- An Efficient Spheroid Manufacturing Workflow for WJ-MSCs: Comparative Evaluation of Immediate 3D Aggregation and Conventional 2D Stabilization.Bioengineering (Basel, Switzerland) · 2026Article
- Amino Acid Uptake Limitations during Human Mesenchymal Stem Cell-Based Chondrogenesis.Tissue engineering. Part A · 2025Article
- Temporal Enzymatic Treatment to Enhance the Remodeling of Multiple Cartilage Microtissues into a Structurally Organized Tissue.Advanced healthcare materials · 2024Article
- Conditioned media derived from human fetal progenitor cells improves skin regeneration in burn wound healing.Cell and tissue research · 2022Article
- The Effects of Transforming Growth Factor-BioMed research international · 2022Article
- Scaffold-Free Engineering of Human Cartilage Implants.Cartilage · 2021Article
- Evaluation of a Cell-Free Collagen Type I-Based Scaffold for Articular Cartilage Regeneration in an Orthotopic Rat Model.Materials (Basel, Switzerland) · 2020Article
- Article
- Cartilage Extracellular Matrix Scaffold With Kartogenin-Encapsulated PLGA Microspheres for Cartilage Regeneration.Frontiers in bioengineering and biotechnology · 2020Article
- [Development of cartilage extracellular matrix in cartilage tissue engineering].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2019Review
- Mesenchymal Stem Cell Migration during Bone Formation and Bone Diseases Therapy.International journal of molecular sciences · 2018Review
- Harnessing extracellular vesicles to direct endochondral repair of large bone defects.Bone & joint research · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCartilage formation from self-assembling mesenchymal stem cells (MSCs) in vitro recapitulate important cellular events during mesenchymal condensation that precedes native cartilage development. The goal of this study was to investigate the effects of cartilaginous extracellular matrix (ECM) components and culture regimen on cartilage formation by self-assembling human MSCs in vitro and in vivo.
methodsHuman bone marrow-derived MSCs (hMSCs) were seeded and compacted in 6.5-mm-diameter transwell inserts with coated (type I, type II collagen) or uncoated (vehicle) membranes, at different densities (0.5 × 10
resultsType I and type II collagen coatings enabled cartilage disc formation from self-assembling hMSCs. Without ECM coating, hMSCs formed dome-shaped tissues resembling the pellets. Type I collagen, expressed in the prechondrogenic mesenchyme, improved early chondrogenesis versus type II collagen. High seeding density improved cartilage tissue properties but resulted in a lower yield of disc formation. Discs and pellets exhibited compositional and organizational differences in vitro and in vivo. Prolonged chondrogenic induction of the discs in vitro expedited endochondral ossification in vivo.
conclusionsThe outcomes of cartilage tissues formed from self-assembling MSCs in vitro and in vivo can be modulated by the control of culture parameters. These insights could motivate new directions for engineering cartilage and bone via a cartilage template from self-assembling MSCs.
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Registered trials
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