ArticleProceedings of the National Academy of Sciences of the United States of America2016
Artemisia annua mutant impaired in artemisinin synthesis demonstrates importance of nonenzymatic conversion in terpenoid metabolism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 91 citations in OpenAlex.
- Regulatory architecture of high-altitude adaptation in Artemisia: from signal perception to specialized metabolism.Plant cell reports · 2026Review
- Single-nucleus transcriptomics reveal the morphogenesis and artemisinin biosynthesis in Artemisia annua glandular trichomes.Nature communications · 2025Article
- Unlocking the potential of3 Biotech · 2025Review
- Engineering scutellarin biosynthesis in Artemisia annua.Plant cell reports · 2025Article
- Comparison of the composition and antiplasmodial activity of Artemisia annua teas using an untargeted metabolomic approach.PloS one · 2025Article
- Impact of elicitors and light on biosynthesis of sesquiterpene lactones in tissue culture ofFrontiers in plant science · 2025Article
- Plant sesquiterpene lactones.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Natural Compounds for the Treatment of Acute Pancreatitis: Novel Anti-Inflammatory Therapies.Biomolecules · 2024Review
- TrichomeLess Regulator 3 is required for trichome initial and cuticle biosynthesis in Artemisia annua.Molecular horticulture · 2024Article
- Advanced metabolic engineering strategies for increasing artemisinin yield inHorticulture research · 2024Article
- Trichome-Specific Analysis and Weighted Gene Co-Expression Correlation Network Analysis (WGCNA) Reveal Potential Regulation Mechanism of Artemisinin Biosynthesis inInternational journal of molecular sciences · 2023Article
- Increased artemisinin production by promoting glandular secretory trichome formation and reconstructing the artemisinin biosynthetic pathway inHorticulture research · 2023Article
- Current Status and De Novo Synthesis of Anti-Tumor Alkaloids inMetabolites · 2023Review
- Article
- Genetic analysis reveals the inconsistency of amorpha-4,11-diene synthase, a key enzyme in the artemisinin synthesis pathway, in asteraceae.Chinese medicine · 2023Article
- Germline transformation ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Article
- Jasmonate- and abscisic acid-activated AaGSW1-AaTCP15/AaORA transcriptional cascade promotes artemisinin biosynthesis in Artemisia annua.Plant biotechnology journal · 2021Article
- Chromatin Accessibility Is Associated with Artemisinin Biosynthesis Regulation inMolecules (Basel, Switzerland) · 2021Article
- Genome-Wide Analysis of Light-Regulated Alternative Splicing inFrontiers in plant science · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Artemisinin, a sesquiterpene lactone produced by Artemisia annua glandular secretory trichomes, is the active ingredient in the most effective treatment for malaria currently available. We identified a mutation that disrupts the amorpha-4,11-diene C-12 oxidase (CYP71AV1) enzyme, responsible for a series of oxidation reactions in the artemisinin biosynthetic pathway. Detailed metabolic studies of cyp71av1-1 revealed that the consequence of blocking the artemisinin biosynthetic pathway is the redirection of sesquiterpene metabolism to a sesquiterpene epoxide, which we designate arteannuin X. This sesquiterpene approaches half the concentration observed for artemisinin in wild-type plants, demonstrating high-flux plasticity in A. annua glandular trichomes and their potential as factories for the production of novel alternate sesquiterpenes at commercially viable levels. Detailed metabolite profiling of leaf maturation time-series and precursor-feeding experiments revealed that nonenzymatic conversion steps are central to both artemisinin and arteannuin X biosynthesis. In particular, feeding studies using
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