Evidence map›Paper›PMID 27929752›Full record

ArticlemAbs

Efficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells.

Michael Dillon, Yiyuan Yin, Jianhui Zhou, Luke McCarty, Diego Ellerman, Dionysos Slaga, Teemu T Junttila, Guanghui Han, Wendy Sandoval, Meric A Ovacik and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 75 citations in OpenAlex.

  1. Article
  2. Generation of Bispecific Antibodies Using FAST-Ig™.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Toward Drug-Like Multispecific Antibodies by Design.International journal of molecular sciences · 2020
    Review
  16. Article
  17. High-throughput antibody screening from complex matrices using intact protein electrospray mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Michael Dillona Department of Antibody Engineering , Genentech, Inc. , South San Francisco , CA , USA.
Yiyuan Yina Department of Antibody Engineering , Genentech, Inc. , South San Francisco , CA , USA.
Jianhui Zhoua Department of Antibody Engineering , Genentech, Inc. , South San Francisco , CA , USA.
Luke McCartyb Department of Protein Chemistry , Genentech, Inc. , South San Francisco , CA , USA.
Diego Ellermanb Department of Protein Chemistry , Genentech, Inc. , South San Francisco , CA , USA.
Dionysos Slagac Department of Translational Oncology , Genentech, Inc. , South San Francisco , CA , USA.
Teemu T Junttilac Department of Translational Oncology , Genentech, Inc. , South San Francisco , CA , USA.
Guanghui Hand Department of Microchemistry, Proteomics and Lipidomics , Genentech, Inc. , South San Francisco , CA , USA.
Wendy Sandovald Department of Microchemistry, Proteomics and Lipidomics , Genentech, Inc. , South San Francisco , CA , USA.
Meric A Ovacike Department of Preclinical and Translational Pharmacokinetics , Genentech, Inc. , South San Francisco , CA , USA.
Kedan Line Department of Preclinical and Translational Pharmacokinetics , Genentech, Inc. , South San Francisco , CA , USA.
Zhilan Huf Department of Early Stage Cell Culture , Genentech, Inc. , South San Francisco , CA , USA.
Amy Shenf Department of Early Stage Cell Culture , Genentech, Inc. , South San Francisco , CA , USA.
Jacob E Corng Department of Early Discovery Biochemistry, Genentech, Inc. , South San Francisco , CA , USA.
Christoph Spiessa Department of Antibody Engineering , Genentech, Inc. , South San Francisco , CA , USA.
Paul J Cartera Department of Antibody Engineering , Genentech, Inc. , South San Francisco , CA , USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bispecific IgG production in single host cells has been a much sought-after goal to support the clinical development of these complex molecules. Current routes to single cell production of bispecific IgG include engineering heavy chains for heterodimerization and redesign of Fab arms for selective pairing of cognate heavy and light chains. Here, we describe novel designs to facilitate selective Fab arm assembly in conjunction with previously described knobs-into-holes mutations for preferential heavy chain heterodimerization. The top Fab designs for selective pairing, namely variants v10 and v11, support near quantitative assembly of bispecific IgG in single cells for multiple different antibody pairs as judged by high-resolution mass spectrometry. Single-cell and in vitro-assembled bispecific IgG have comparable physical, in vitro biological and in vivo pharmacokinetics properties. Efficient single-cell production of bispecific IgG was demonstrated for human IgG

Indexed as

AnimalsAntibodies, BispecificHumansImmunoglobulin GProtein EngineeringAntibodies, BispecificImmunoglobulin GBispecific antibodybispecific IgGorthogonal Fab engineeringsingle-cell productionstable CHO cell lines

Identifiers

PMID27929752
PMCPMC5297516
OpenAlexW2560421980

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.