ArticleCell cycle (Georgetown, Tex.)2017
Genome-wide screen of cell-cycle regulators in normal and tumor cells identifies a differential response to nucleosome depletion.
Article in Cell cycle (Georgetown, Tex.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Human CRAMP1 specifically promotes the expression of histone H1 genes.EMBO reports · 2026Article
- PRMT5 activity sustains histone production to maintain genome integrity.bioRxiv : the preprint server for biology · 2025Article
- Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality.Cell genomics · 2025Article
- Robust p53 phenotypes and prospective downstream targets in telomerase-immortalized human cells.Oncotarget · 2025Article
- Acute multi-level response to defectivebioRxiv : the preprint server for biology · 2024Article
- Cyclin E/CDK2 and feedback from soluble histone protein regulate the S phase burst of histone biosynthesis.Cell reports · 2023Article
- Article
- An Expanded Interplay Network between NF-κB p65 (RelA) and E2F1 Transcription Factors: Roles in Physiology and Pathology.Cancers · 2022Article
- Hinfp is a guardian of the somatic genome by repressing transposable elements.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Landscape of functional interactions of human processive ribonucleases revealed by high-throughput siRNA screenings.iScience · 2021Article
- Reduce, Retain, Recycle: Mechanisms for Promoting Histone Protein Degradation versus Stability and Retention.Molecular and cellular biology · 2021Review
- High-content imaging-based pooled CRISPR screens in mammalian cells.The Journal of cell biology · 2021Article
- Functional annotation of human long noncoding RNAs via molecular phenotyping.Genome research · 2020Article
- ChromID identifies the protein interactome at chromatin marks.Nature biotechnology · 2020Article
- CDK11 is required for transcription of replication-dependent histone genes.Nature structural & molecular biology · 2020Article
- Specificity landscapes unmask submaximal binding site preferences of transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Contribution of allelic imbalance to colorectal cancer.Nature communications · 2018Article
- CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.Nature medicine · 2018Article
- QuaDMutEx: quadratic driver mutation explorer.BMC bioinformatics · 2017Article
- Essential role for centromeric factors following p53 loss and oncogenic transformation.Genes & development · 2017Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify cell cycle regulators that enable cancer cells to replicate DNA and divide in an unrestricted manner, we performed a parallel genome-wide RNAi screen in normal and cancer cell lines. In addition to many shared regulators, we found that tumor and normal cells are differentially sensitive to loss of the histone genes transcriptional regulator CASP8AP2. In cancer cells, loss of CASP8AP2 leads to a failure to synthesize sufficient amount of histones in the S-phase of the cell cycle, resulting in slowing of individual replication forks. Despite this, DNA replication fails to arrest, and tumor cells progress in an elongated S-phase that lasts several days, finally resulting in death of most of the affected cells. In contrast, depletion of CASP8AP2 in normal cells triggers a response that arrests viable cells in S-phase. The arrest is dependent on p53, and preceded by accumulation of markers of DNA damage, indicating that nucleosome depletion is sensed in normal cells via a DNA-damage -like response that is defective in tumor cells.
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