ArticleJournal of visualized experiments : JoVE2016
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- A systems toxicology approach implicates post-transcriptional regulatory networks in reproductive defects from PFAS exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- ERK activation dynamics in maturing oocyte controls embryonic nuclear divisions in Caenorhabditis elegans.Cell reports · 2025Article
- Polyhydroxybutyrate production by freshwater SAR11 (LD12).The ISME journal · 2025Article
- Article
- Pachytene piRNAs control discrete meiotic events during spermatogenesis and restrict gene expression in space and time.Science advances · 2024Article
- Assessment of the FRET-based Teen sensor to monitor ERK activation changes preceding morphological defects in a RASopathy zebrafish model and phenotypic rescue by MEK inhibitor.Molecular medicine (Cambridge, Mass.) · 2024Article
- Mitochondrial GTP metabolism controls reproductive aging in C. elegans.Developmental cell · 2023Article
- Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.Cell reports · 2023Article
- Two distinct mechanisms lead to either oocyte or spermatocyte decrease in C. elegans after whole developmental exposure to γ-rays.PloS one · 2023Article
- C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining.Journal of visualized experiments : JoVE · 2022Article
- TUNEL Labeling to Detect Double-stranded DNA Breaks inBio-protocol · 2022Article
- Reevaluation of the role of LIP-1 as an ERK/MPK-1 dual specificity phosphatase in theProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline.Genetics · 2021Article
- ERK phosphorylates chromosomal axis component HORMA domain protein HTP-1 to regulate oocyte numbers.Science advances · 2020Article
- Antitumoral Drug Potential of Tryptophan-Betaxanthin and Related Plant Betalains in theAntioxidants (Basel, Switzerland) · 2020Article
- In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay.Journal of visualized experiments : JoVE · 2020Article
- Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.Nature communications · 2018Article
- Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The evolutionarily conserved extracellular signal transducing RTK-RAS-ERK pathway is an important kinase-signaling cascade that controls multiple cellular and developmental processes principally via activation of ERK, the terminal kinase of the pathway. Tight regulation of ERK activity is essential for normal development and homeostasis; overly active ERK results in excessive cellular proliferation, while underactive ERK causes cell death. C. elegans is a powerful model system that has helped characterize the function and regulation of RTK-RAS-ERK signaling pathway during development. In particular, the RTK-RAS-ERK pathway is essential for C. elegans germline development, which is the focus of this method. Using antibodies specific to the active, diphosphorylated form of ERK (dpERK), the stereotypical localization pattern can be visualized within the germline. Because this pattern is both spatially and temporally controlled, the ability to reproducibly assay dpERK is useful to identify regulators of the pathway that affect dpERK signal duration and amplitude and thus germline development. Here we demonstrate how to successfully dissect, stain, and image dpERK within the C. elegans gonad. This method can be adapted for spatial localization of any signaling or structural protein in the C. elegans gonad, provided an antibody compatible with immunofluorescence is available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.