ArticleScientific reports2016
RNAi-mediated mortality of the whitefly through transgenic expression of double-stranded RNA homologous to acetylcholinesterase and ecdysone receptor in tobacco plants.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 96 citations in OpenAlex.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential.Plants (Basel, Switzerland) · 2026Review
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- Novel dsRNA designs increase the stability and bioefficacy of RNAi-based pesticides forFrontiers in insect science · 2026Article
- Metabolomic profiling of VOC-driven interactions between Priestia megaterium and Bacillus licheniformis in a simulated rhizosphere using split petri dishes.Archives of microbiology · 2025Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Neurotoxicity, Cytotoxicity, and Genotoxicity of Phyto-radio Synthesized Selenium Nanoparticles in Culex pipiens Complex.Biological trace element research · 2025Article
- Characterization of a uridine diphosphate (UDP)-glycosyltransferase gene associated with abamectin resistance in two-spotted spider mite, Tetranychus urticae.Experimental & applied acarology · 2025Article
- Horizontally transferred genes as RNA interference targets for aphid and whitefly control.Plant biotechnology journal · 2023Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- RNA interference-mediated knockdown of genes involved in sugar transport and metabolism disrupts psyllidFrontiers in insect science · 2023Article
- Plant resistance against whitefly and its engineering.Frontiers in plant science · 2023Review
- RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management.Pest management science · 2022Review
- Foliar application of clay-delivered RNA interference for whitefly control.Nature plants · 2022Article
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides.Frontiers in bioengineering and biotechnology · 2022Review
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The whitefly Bemisia tabaci (Genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. Using RNA interference (RNAi) to down regulate whitefly genes by expressing their homologous double stranded RNAs in plants has great potential for management of whiteflies to reduce plant virus disease spread. Using a Tobacco rattle virus-derived plasmid for in planta transient expression of double stranded RNA (dsRNA) homologous to the acetylcholinesterase (AChE) and ecdysone receptor (EcR) genes of B. tabaci, resulted in significant adult whitefly mortality. Nicotiana tabacum L. plants expressing dsRNA homologous to B. tabaci AChE and EcR were constructed by fusing sequences derived from both genes. Mortality of adult whiteflies exposed to dsRNA by feeding on N. tabacum plants, compared to non-dsRNA expressing plants, recorded at 24-hr intervals post-ingestion for three days, was >90% and 10%, respectively. Analysis of gene expression by real time quantitative PCR indicated that whitefly mortality was attributable to the down-regulation of both target genes by RNAi. Results indicated that knock down of whitefly genes involved in neuronal transmission and transcriptional activation of developmental genes, has potential as a bio-pesticide to reduce whitefly population size and thereby decrease virus spread.
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