ArticleMicrobial biotechnology2017
Intracellular metabolite profiling of Saccharomyces cerevisiae evolved under furfural.
Article in Microbial biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.Biotechnology letters · 2024Article
- Furfural tolerance of mutant Saccharomyces cerevisiae selected via ionizing radiation combined with adaptive laboratory evolution.Biotechnology for biofuels and bioproducts · 2024Article
- Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistantFrontiers in microbiology · 2023Article
- Candida glycerinogenes Strains Overexpressing Transcription Factors have Improved Furfural Tolerance in Ethanol Production from Non-detoxified Cellulose Hydrolysate.Current microbiology · 2022Article
- Toxic effect and inability of L-homoserine to be a nitrogen source for growth ofFrontiers in microbiology · 2022Article
- Large dependency of intracellular NAD and CoA pools on cultivation conditions in Saccharomyces cerevisiae.BMC research notes · 2021Article
- Reasons for 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde resistance in Saccharomyces cerevisiae: current state of knowledge and perspectives for further improvements.Applied microbiology and biotechnology · 2021Review
- Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae.Microbial cell factories · 2021Article
- Metabolic Changes Induced by Deletion of Transcriptional RegulatorMicroorganisms · 2020Article
- Pathway-based signature transcriptional profiles as tolerance phenotypes for the adapted industrial yeast Saccharomyces cerevisiae resistant to furfural and HMF.Applied microbiology and biotechnology · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Intracellular metabolite profiling of Saccharomyces cerevisiae evolved under furfural.Microbial biotechnology · 2017Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Furfural, one of the most common inhibitors in pre-treatment hydrolysates, reduces the cell growth and ethanol production of yeast. Evolutionary engineering has been used as a selection scheme to obtain yeast strains that exhibit furfural tolerance. However, the response of Saccharomyces cerevisiae to furfural at the metabolite level during evolution remains unknown. In this study, evolutionary engineering and metabolomic analyses were applied to determine the effects of furfural on yeasts and their metabolic response to continuous exposure to furfural. After 50 serial transfers of cultures in the presence of furfural, the evolved strains acquired the ability to stably manage its physiological status under the furfural stress. A total of 98 metabolites were identified, and their abundance profiles implied that yeast metabolism was globally regulated. Under the furfural stress, stress-protective molecules and cofactor-related mechanisms were mainly induced in the parental strain. However, during evolution under the furfural stress, S. cerevisiae underwent global metabolic allocations to quickly overcome the stress, particularly by maintaining higher levels of metabolites related to energy generation, cofactor regeneration and recovery from cellular damage. Mapping the mechanisms of furfural tolerance conferred by evolutionary engineering in the present study will be led to rational design of metabolically engineered yeasts.
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