ArticleJournal of clinical microbiology2017
Zika Virus Testing Considerations: Lessons Learned from the First 80 Real-Time Reverse Transcription-PCR-Positive Cases Diagnosed in New York State.
Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Detectability of Pathogenic RNA Virus Families in Different Body Sites: A Scoping Review.Viruses · 2026Article
- Novel Therapeutic Nutrients Molecules That Protect against Zika Virus Infection with a Special Note on Palmitoleate.Nutrients · 2022Review
- A fatal case report of antibody-dependent enhancement of dengue virus type 1 following remote Zika virus infection.BMC infectious diseases · 2021Article
- Evaluating Differences in Whole Blood, Serum, and Urine Screening Tests for Zika Virus, Puerto Rico, USA, 2016.Emerging infectious diseases · 2021Article
- Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology.Emerging infectious diseases · 2021Article
- Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research.PloS one · 2021Article
- Comparative specificity and sensitivity of NS1-based serological assays for the detection of flavivirus immune response.PLoS neglected tropical diseases · 2020Article
- Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays.Clinical microbiology reviews · 2019Review
- Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.Insect biochemistry and molecular biology · 2019Article
- Experimental Zika virus infection of Jamaican fruit bats (Artibeus jamaicensis) and possible entry of virus into brain via activated microglial cells.PLoS neglected tropical diseases · 2019Article
- Review
- Zika: An emerging disease requiring prevention and awareness.PLoS neglected tropical diseases · 2018Article
- Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses.Nature communications · 2018Article
- Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.Scientific reports · 2018Article
- Diagnosis of Zika Virus Infections: Challenges and Opportunities.The Journal of infectious diseases · 2017Review
- Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification.Scientific reports · 2017Article
- Update: Interim Guidance for Health Care Providers Caring for Pregnant Women with Possible Zika Virus Exposure - United States (Including U.S. Territories), July 2017.MMWR. Morbidity and mortality weekly report · 2017Article
- Antibody Responses to Zika Virus Infections in Environments of Flavivirus Endemicity.Clinical and vaccine immunology : CVI · 2017Article
- Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
The performance and interpretation of laboratory tests for Zika virus (ZKV) continue to be evaluated. Serology is cross-reactive, laborious, and frequently difficult to interpret, and serum was initially solely recommended for molecular diagnosis. ZKV testing was initiated in January 2016 in New York State for symptomatic patients, pregnant women, their infants, and patients with Guillain-Barré syndrome who had traveled to areas with ZKV transmission. Subsequently, eligibility was expanded to pregnant women with sexual partners with similar travel histories. Serum and urine collected within 4 weeks of symptom onset or within 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes. In this review of lessons learned from the first 80 positive cases in NYS, ZKV RNA was detected in urine only in 50 patients, in serum only in 19 patients, and in both samples concurrently in 11 patients, with average viral loads in urine a log higher than those in serum. Among 93 positive samples from the 80 patients, 41 were positive on both gene assays, 52 were positive on the envelope only, and none were positive on the NS2B only. Of the 80 infected patients, test results for 74 (93%) would have defined their infection status as not detected or equivocal if the requirement for positive results from two assay targets (two-target-positive requirement) in the initial federal guidance to public health laboratories was enforced, if urine was not tested, or if the extended eligibility time for molecular testing was not implemented. These changes facilitated more extensive molecular diagnosis of ZKV, reducing reliance on time-consuming and potentially inconclusive serology.
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