Evidence map›Paper›PMID 27927228›Full record

ArticleBMC research notes2016

Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL.

Vincent Pujol, Jose Robles, Penghao Wang, Jen Taylor, Peng Zhang, Li Huang, Linda Tabe, Evans Lagudah

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Blocked at the Stomatal Gate, a Key Step of WheatFrontiers in plant science · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Vincent PujolCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia. pujol.vct@gmail.com.ORCID http://orcid.org/0000-0001-9903-3475
Jose RoblesCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia.
Penghao WangCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia.
Jen TaylorCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia.
Peng ZhangPlant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, 2570, Australia.
Li HuangDepartment of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717, USA.
Linda TabeCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia.
Evans LagudahCSIRO Agriculture and Food, GPO Box 1600, Canberra, ACT, 2601, Australia.
Commonwealth Scientific and Industrial Research Organisation · AUMontana State University · USThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their mechanisms of action is often lacking. In this study, the mechanism of resistance conferred by a recently discovered stem rust resistance locus on wheat chromosome 7AL was investigated through microscopic observations and RNA-sequencing, using the susceptible line Columbus and the independent, backcrossed, resistant lines Columbus-NS765 and Columbus-NS766.

resultsMicroscopic observations of infected leaves revealed that the resistance conferred by the 7AL resistance locus was initiated 2 days post-inoculation, upon the fungus entry into the plant through the stoma. Resistance was manifested by death of guard and epidermal cells adjacent to an infection site. Occasionally, similar observations were made in the susceptible line, suggesting that the resistance response was the same in all genotypes, but enhanced in the resistant lines. Transcriptomic analysis, combined with assignment of genes to wheat chromosomes, revealed a disproportionately high number of differentially expressed genes were located on chromosomes 7AL and 6A. A number of genes annotated as cysteine-rich receptor-like kinases were located on chromosome 7AL. Closer investigation indicated that the encoded proteins were in fact putative receptor-like cytoplasmic kinases. One of the putative RLCK genes contained a SNP marker previously shown to co-segregate with the 7AL resistance locus. The results also indicated the presence of a large introgression on chromosome 6A in both resistant lines, but whether it has any role in the resistance response is unclear.

conclusionsThis study represents the first investigation on the resistance mechanism conferred by the wheat 7AL stem rust resistance locus. The resistance response was associated with pre-haustorial cell death, and the transcriptome analysis suggested putative receptor-like cytoplasmic kinases as candidate resistance genes for further investigation.

Indexed as

BasidiomycotaChromosome MappingChromosomes, PlantCyclopentanesDisease ResistanceFluorescein-5-isothiocyanateGenes, PlantOxylipinsPhenotypePlant DiseasesPolymorphism, Single NucleotideSalicylic AcidSequence Analysis, RNASignal TransductionTranscriptomeTriticumCyclopentanesFluorescein-5-isothiocyanatejasmonic acidOxylipinsSalicylic Acid7DL suppressorRNA-SeqStem rustUg99, 7AL resistanceWheat

Identifiers

PMID27927228
PMCPMC5143453
OpenAlexW2560382459

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.