ArticleScientific reports2016
Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Microfluidic Electroporation Arrays for Investigating Electroporation-Induced Cellular Rupture Dynamics.Biosensors · 2024Article
- Biomaterial-based gene therapy.MedComm · 2023Review
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Stability of the nitroxide biradical AMUPol in intact and lysed mammalian cells.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2022Article
- Microfluidic mechanoporation for cellular delivery and analysis.Materials today. Bio · 2022Review
- In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells.Journal of the American Chemical Society · 2021Article
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.Biotechnology letters · 2020Article
- Biosensors Based on Mechanical and Electrical Detection Techniques.Sensors (Basel, Switzerland) · 2020Review
- Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.Scientific reports · 2019Article
- Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- Tracking the Evolution of Transiently Transfected Individual Cells in a Microfluidic Platform.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Electroporation serves as a promising non-viral gene delivery approach, while its current configuration carries several drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here we developed a new micropillar array electroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes into mammalian cells. By introducing well-patterned micropillar array texture on the electrode surface, the number of pillars each cell faces varies with its plasma membrane surface area, despite their large population and random locations. In this way, cell size specific electroporation is conveniently carried out, contributing to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10-55% transgene knockdown with siRNA probes, respectively. The delivery efficiency varies with the number and size of micropillars as well as their pattern density. As MAE works like many single cell electroporation are carried out in parallel, the electrophysiology response of individual cells is representative, which has potentials to facilitate the tedious, cell-specific protocol screening process in current bulk electroporation (i.e., electroporation to a large population of cells). Its success might promote the wide adoption of electroporation as a safe and effective non-viral gene delivery approach needed in many biological research and clinical treatments.
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Registered trials
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