ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2016
PREditOR: a synthetic biology approach to removing heterochromatin from cells.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Trial
- Sperm H3K9me3 levels are associated with embryo developmental dynamics and biochemical pregnancy in IVF patients with normozoospermia.Reproductive biology and endocrinology : RB&E · 2025Article
- Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.Epigenetics & chromatin · 2021Article
- Induction of spontaneous human neocentromere formation and long-term maturation.The Journal of cell biology · 2021Article
- Large domains of heterochromatin direct the formation of short mitotic chromosome loops.eLife · 2020Article
- H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.Journal of cell science · 2020Article
- Generation of a Synthetic Human Chromosome with Two Centromeric Domains for Advanced Epigenetic Engineering Studies.ACS synthetic biology · 2018Article
- HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.The EMBO journal · 2018Article
- Review
- Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase.PloS one · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
It is widely accepted that heterochromatin is necessary to maintain genomic stability. However, direct experimental evidence supporting this is slim. Previous studies using either enzyme inhibitors, gene knockout or knockdown studies all are subject to the caveat that drugs may have off-target effects and enzymes that modify chromatin proteins to support heterochromatin formation may also have numerous other cellular targets as well. Here, we describe PREditOR (protein reading and editing of residues), a synthetic biology approach that allows us to directly remove heterochromatin from cells without either drugs or global interference with gene function. We find that removal of heterochromatin perturbs mitotic progression and causes a dramatic increase in chromosome segregation defects, possibly as a result of interfering with the normal centromeric localization of the chromosomal passenger complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.