ArticleBiotechnology for biofuels2016
Increasing n-butanol production with
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 66 citations in OpenAlex.
- Optimizing hexanoic acid biosynthesis in Saccharomyces cerevisiae for the de novo production of olivetolic acid.Biotechnology for biofuels and bioproducts · 2024Article
- A comparative analysis of NADPH supply strategies inMetabolic engineering communications · 2024Article
- Towards biotechnological production of bio-based low molecular weight esters: a patent review.RSC advances · 2024Review
- The response mechanism analysis ofFrontiers in microbiology · 2024Article
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- An optimized reverse β-oxidation pathway to produce selected medium-chain fatty acids in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway.Journal of microbiology (Seoul, Korea) · 2022Article
- Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and β-alanine metabolism.Microbial biotechnology · 2022Article
- Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.World journal of microbiology & biotechnology · 2022Article
- Glucose/Xylose Co-FermentingFrontiers in bioengineering and biotechnology · 2022Article
- Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2021Article
- n-Butanol production by Rhodopseudomonas palustris TIE-1.Communications biology · 2021Article
- An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts.Nature communications · 2021Article
- Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides.Microbial cell factories · 2021Review
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
- Vitamin requirements and biosynthesis in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2020Review
- Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges.World journal of microbiology & biotechnology · 2020Review
- Modulating acetate ester and higher alcohol production in Saccharomyces cerevisiae through the cofactor engineering.Journal of industrial microbiology & biotechnology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundn-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some
resultsIn our recent work, we showed that n-butanol production via a clostridial acetoacetyl-CoA-derived pathway in engineered yeast was limited by the availability of coenzyme A (CoA) and cytosolic acetyl-CoA. Increasing their levels resulted in a strain producing up to 130 mg/L n-butanol under anaerobic conditions. Here, we show that under aerobic conditions. this strain can even produce up to 235 mg/L n-butanol probably due to a more efficient NADH re-oxidation. Nevertheless, expression of a bacterial water-forming NADH oxidase (nox) significantly reduced n-butanol production although it showed a positive effect on growth and glucose consumption. Screening for an improved version of an acetyl-CoA forming NAD
conclusionsBy further optimizing substrate supply and redox power in the form of coenzyme A, acetyl-CoA and NADH, n-butanol production with engineered yeast cells could be improved to levels never reached before with
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.