Evidence map›Paper›PMID 27923980›Full record

Trial reportPhysiological reports2016

Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism.

Eva-Karin Gidlund, Ferdinand von Walden, Mika Venojärvi, Ulf Risérus, Olli J Heinonen, Jessica Norrbom, Carl Johan Sundberg

Open access · goldAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Physiological reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 3 pooled it
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 3 syntheses or guidelines pooled it, 57 citations in OpenAlex.

  1. Pooled it
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  9. Article
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  14. Exercise attenuates the hallmarks of aging: Novel perspectives.Journal of sport and health science · 2025
    Review
  15. Article
  16. Review
  17. Review
  18. Impaired Expression of Humanin during Adrenocortical Carcinoma.International journal of molecular sciences · 2024
    Article
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Eva-Karin GidlundDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden eva-karin.gidlund@ki.se.
Ferdinand von WaldenNeuropediatrics Unit, Department of Women's and Children's Health, Karolinska Institutet and Astrid Lindgren's Pediatric Hospital, Stockholm, Sweden.
Mika VenojärviInstitute of Biomedicine, Sports and exercise medicine, University of Eastern Finland, Kuopio, Finland.
Ulf RisérusClinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Uppsala University, Uppsala, Sweden.
Olli J HeinonenPaavo Nurmi Centre and Departmen of Health & Physical Activity, University of Turku, Turku, Finland.
Jessica NorrbomDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Carl Johan SundbergDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Karolinska Institutet · SEPaavo Nurmen Säätiö · FIUniversity of Eastern Finland · FIUppsala University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Humanin (HN) is a mitochondrially encoded and secreted peptide linked to glucose metabolism and tissue protecting mechanisms. Whether skeletal muscle HN gene or protein expression is influenced by exercise remains unknown. In this intervention study we show, for the first time, that HN protein levels increase in human skeletal muscle following 12 weeks of resistance training in persons with prediabetes. Male subjects (n = 55) with impaired glucose regulation (IGR) were recruited and randomly assigned to resistance training, Nordic walking or a control group. The exercise interventions were performed three times per week for 12 weeks with progressively increased intensity during the intervention period. Biopsies from the vastus lateralis muscle and venous blood samples were taken before and after the intervention. Skeletal muscle and serum protein levels of HN were analyzed as well as skeletal muscle gene expression of the mitochondrially encoded gene MT-RNR2, containing the open reading frame for HN To elucidate mitochondrial training adaptation, mtDNA, and nuclear DNA as well as Citrate synthase were measured. Skeletal muscle HN protein levels increased by 35% after 12 weeks of resistance training. No change in humanin protein levels was seen in serum in any of the intervention groups. There was a significant correlation between humanin levels in serum and the improvements in the 2 h glucose loading test in the resistance training group. The increase in HN protein levels in skeletal muscle after regular resistance training in prediabetic males may suggest a role for HN in the regulation of glucose metabolism. Given the preventative effect of exercise on diabetes type 2, the role of HN as a mitochondrially derived peptide and an exercise-responsive mitokine warrants further investigation.

Indexed as

Resistance TrainingAdultBlood GlucoseCitrate (si)-SynthaseFemaleHumansIntracellular Signaling Peptides and ProteinsMaleMiddle AgedMitochondria, MuscleMuscle, SkeletalPrediabetic StateBlood GlucoseCitrate (si)-SynthasehumaninIntracellular Signaling Peptides and ProteinsExercisehumanhumaninimpaired glucose regulationskeletal muscle

Identifiers

PMID27923980
PMCPMC5357820
OpenAlexW2560092842

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.