ArticlemBio2016
Attenuation of the Type IV Pilus Retraction Motor Influences Neisseria gonorrhoeae Social and Infection Behavior.
Article in mBio, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- The stoichiometry of minor-to-major pilins regulates the dynamic activity of the type IVa competence pilus in Vibrio cholerae.PLoS genetics · 2026Article
- Hyperpiliation, not loss of pilus retraction, reducesMicrobiology spectrum · 2025Article
- Neisseria gonorrhoeae induces local secretion of IL-10 at the human cervix to promote colonization.The Journal of clinical investigation · 2024Article
- Diclofenac sensitizes multi-drug resistant Acinetobacter baumannii to colistin.PLoS pathogens · 2024Article
- A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation.BMC microbiology · 2024Article
- Type IV pilus retraction is required formBio · 2024Article
- Multisite transformation inFrontiers in microbiology · 2023Article
- The PilT retraction ATPase promotes both extension and retraction of the MSHA type IVa pilus in Vibrio cholerae.PLoS genetics · 2022Article
- Gonococcal invasion into epithelial cells depends on both cell polarity and ezrin.PLoS pathogens · 2021Article
- Motor-independent retraction of type IV pili is governed by an inherent property of the pilus filament.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Fresh Extension of Vibrio cholerae Competence Type IV Pili Predisposes Them for Motor-Independent Retraction.Applied and environmental microbiology · 2021Article
- Article
- Type IV pilus retraction enables sustained bacteremia and plays a key role in the outcome of meningococcal sepsis in a humanized mouse model.PLoS pathogens · 2021Article
- A bifunctional ATPase drives tad pilus extension and retraction.Science advances · 2019Article
- PilT and PilU are homohexameric ATPases that coordinate to retract type IVa pili.PLoS genetics · 2019Article
- Motor Properties of PilT-Independent Type 4 Pilus Retraction in Gonococci.Journal of bacteriology · 2019Article
- Type IV pili: dynamics, biophysics and functional consequences.Nature reviews. Microbiology · 2019Review
- Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.Biophysical journal · 2019Article
- Genus-Wide Comparative Genomics Analysis ofInternational journal of genomics · 2019Article
- Perturbing the acetylation status of the Type IV pilus retraction motor, PilT, reduces Neisseria gonorrhoeae viability.Molecular microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Retraction of the type IV pilus (Tfp) mediates DNA uptake, motility, and social and infection behavior in a wide variety of prokaryotes. To date, investigations into Tfp retraction-dependent activities have used a mutant deleted of PilT, the ATPase motor protein that causes the pilus fiber to retract. ΔpilT cells are nontransformable, nonmotile, and cannot aggregate into microcolonies. We tested the hypothesis that these retraction-dependent activities are sensitive to the strength of PilT enzymatic activity by using the pathogen Neisseria gonorrhoeae as a model. We constructed an N. gonorrhoeae mutant with an amino acid substitution in the PilT Walker B box (a substitution of cysteine for leucine at position 201, encoded by pilT IMPORTANCE: Type IV pili are fibers expressed on the surface of many bacteria. Neisseria gonorrhoeae cells crawl, take up DNA, and communicate with each other and with human cells by retracting these fibers. Here, we show that an N. gonorrhoeae mutant expressing an enzymatically weakened type IV pilus retraction motor still crawls and takes up DNA normally. However, mutant cells exhibit abnormal social behavior, and they are less infective because they fail to activate the epidermal growth factor receptor. Our study shows that N. gonorrhoeae social and infection behaviors are sensitive to the strength of the retraction motor enzyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.