Evidence map›Paper›PMID 27923600›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2017

Determinants of orofacial clefting II: Effects of 5-Aza-2'-deoxycytidine on gene methylation during development of the first branchial arch.

Ratnam S Seelan, Partha Mukhopadhyay, Dennis R Warner, Irina A Smolenkova, M Michele Pisano, Robert M Greene

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ratnam S SeelanDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: rsseel01@louisville.edu.
Partha MukhopadhyayDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: p0mukh01@louisville.edu.
Dennis R WarnerDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: dwarn01@louisville.edu.
Irina A SmolenkovaDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: irina.smolenkova@louisville.edu.
M Michele PisanoDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: drmikeky@gmail.com.
Robert M GreeneDepartment of Molecular, Cellular and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY 40202, USA. Electronic address: dr.bob.greene@gmail.com.
University of Louisville · US

Funding

Molecular Determinants of Developmental DefectsP20GM103453 · NIGMS · UNIVERSITY OF LOUISVILLE · PI GREENE, ROBERT M · 2012 to 2012
$1.7M
Transcriptional Coactivators and Pregnancy OutcomesR01HD053509 · NICHD · UNIVERSITY OF LOUISVILLE · PI GREENE, ROBERT M · 2008 to 2012
$1.5M
NUTRITIONAL EPIGENETICS AND OROFACIAL DEVELOPMENTR01DE018215 · NIDCR · UNIVERSITY OF LOUISVILLE · PI GREENE, ROBERT M · 2008 to 2011
$1.3M
Integrated Analysis: Epigenetic Regulation of Gene Expression During Orofacial DeR03DE021460 · NIDCR · UNIVERSITY OF LOUISVILLE · PI BROCK, GUY, MUKHOPADHYAY, PARTHA · 2012 to 2013
$440k
NICHD NIH HHS R01 HD053509NIDCR NIH HHS R01 DE018215NIDCR NIH HHS R03 DE021460NIGMS NIH HHS P20 GM103453
6 · The paper itself

Abstract

Defects in development of the secondary palate, which arise from the embryonic first branchial arch (1-BA), can cause cleft palate (CP). Administration of 5-Aza-2'-deoxycytidine (AzaD), a demethylating agent, to pregnant mice on gestational day 9.5 resulted in complete penetrance of CP in fetuses. Several genes critical for normal palatogenesis were found to be upregulated in 1-BA, 12h after AzaD exposure. MethylCap-Seq (MCS) analysis identified several differentially methylated regions (DMRs) in DNA extracted from AzaD-exposed 1-BAs. Hypomethylated DMRs did not correlate with the upregulation of genes in AzaD-exposed 1-BAs. However, most DMRs were associated with endogenous retroviral elements. Expression analyses suggested that interferon signaling was activated in AzaD-exposed 1-BAs. Our data, thus, suggest that a 12-h in utero AzaD exposure demethylates and activates endogenous retroviral elements in the 1-BA, thereby triggering an interferon-mediated response. This may result in the dysregulation of key signaling pathways during palatogenesis, causing CP.

Indexed as

AnimalsAzacitidineBranchial RegionCleft PalateDecitabineDNA MethylationEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGestational AgeMice, Inbred ICRPregnancyAzacitidineDecitabine5-Aza-2′deoxycytidineCleft palateDNA methylationFirst branchial archMethylCap-Seq

Identifiers

PMID27923600
PMCPMC5303139
OpenAlexW2560696880

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.