ArticleMicrobial cell factories2016
Microbial production of next-generation stevia sweeteners.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Development of In Vitro and In Planta Strategies for Efficient Production of High-Value Steviol Glycosides.Plant biotechnology journal · 2026Article
- Review
- Raman Spectroscopic Authentication of Rebaudioside M: Discriminating Natural, Fermentation-Derived, and Enzymatically Bioconverted Stevia Sweeteners.Foods (Basel, Switzerland) · 2026Article
- Multi-Enzyme Cascade Reaction of Crude Enzyme Strategy for the Economical and Efficient Bioconversion of Rebaudioside A to Rebaudioside M.Biotechnology journal · 2026Article
- -Identification of QTL for steviol glycoside biosynthesis using a SNP-based genetic linkage map for Stevia rebaudiana.G3 (Bethesda, Md.) · 2026Article
- Functional study and sequence variation of the SrUGT85C2 genes in Stevia rebaudiana.BMC plant biology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies.Plants (Basel, Switzerland) · 2026Review
- Discovery of Novel Minor Steviol Glycoside from theBiomolecules · 2026Article
- DyeDactic workflow to predict halochromism of biosynthetic colourants.Communications chemistry · 2026Article
- Advances in semi-rational design of terpene synthases and their modifying enzymes.AMB Express · 2025Review
- Characterization ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhanced Production of Rebaudioside D and Rebaudioside M through V155T Substitution in the Glycosyltransferase UGT91D2 fromJournal of agricultural and food chemistry · 2025Article
- Identification of a 301 bp promoter core region of the SrUGT91D2 gene from Stevia rebaudiana that contributes to hormone and abiotic stress inducibility.BMC plant biology · 2024Article
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Advances in Engineering Nucleotide Sugar Metabolism for Natural Product Glycosylation inACS synthetic biology · 2024Review
- Rational Design for the Complete Synthesis of Stevioside inMicroorganisms · 2024Article
- Transcriptomic Analyses Reveal Insights into the Shared Regulatory Network of Phenolic Compounds and Steviol Glycosides inInternational journal of molecular sciences · 2024Article
- Rebaudioside D decreases adiposity and hepatic lipid accumulation in a mouse model of obesity.Scientific reports · 2024Article
- Selective synthesis of rebaudioside M2 through structure-guided engineering of glycosyltransferase UGT94D1.Frontiers in bioengineering and biotechnology · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively, and have low sweetness thresholds, high maximum sweet intensities and exhibit a greatly reduced lingering bitter taste compared to stevioside and rebaudioside A, the most abundant steviol glucosides in the leaves of Stevia rebaudiana.
resultsIn the metabolic glycosylation grid leading to production of Reb D and Reb M, UGT76G1 was found to catalyze eight different reactions all involving 1,3-glucosylation of steviol C
conclusionsScreening of the mutant library identified mutations with positive impact on the accumulation of Reb D and Reb M. The effect of the introduced mutations on other reactions in the metabolic grid was characterized. This screen made it possible to identify variants, such as UGT76G1
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