ArticleeLife2016
Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Histone methyltransferase DOT1L maintains cell state and restricts cytotoxic potential of CD8 T cells.Science advances · 2025Article
- Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response.Nature communications · 2025Article
- Adenosine kinase inhibition protects mice from abdominal aortic aneurysm via epigenetic modulation of VSMC inflammation.Cardiovascular research · 2024Article
- N-terminal acetylation of Set1-COMPASS fine-tunes H3K4 methylation patterns.Science advances · 2024Article
- The Chromatin Landscape around DNA Double-Strand Breaks in Yeast and Its Influence on DNA Repair Pathway Choice.International journal of molecular sciences · 2023Review
- SETD2: from chromatin modifier to multipronged regulator of the genome and beyond.Cellular and molecular life sciences : CMLS · 2022Review
- Epi-Decoder: Decoding the Local Proteome of a Genomic Locus by Massive Parallel Chromatin Immunoprecipitation Combined with DNA-Barcode Sequencing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies.Scientific reports · 2021Article
- Inhibition of transcription leads to rewiring of locus-specific chromatin proteomes.Genome research · 2020Article
- Strategy for Development of Site-Specific Ubiquitin Antibodies.Frontiers in chemistry · 2020Article
- Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription.The Journal of biological chemistry · 2019Article
- Nucleosome and ubiquitin position Set2 to methylate H3K36.Nature communications · 2019Article
- Conserved crosstalk between histone deacetylation and H3K79 methylation generates DOT1L-dose dependency in HDAC1-deficient thymic lymphoma.The EMBO journal · 2019Article
- Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution.ACS synthetic biology · 2018Article
- Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.Nucleic acids research · 2018Article
- Decoding the chromatin proteome of a single genomic locus by DNA sequencing.PLoS biology · 2018Article
- Dot1 regulates nucleosome dynamics by its inherent histone chaperone activity in yeast.Nature communications · 2018Article
- Histone methylation in DNA repair and clinical practice: new findings during the past 5-years.Journal of Cancer · 2018Review
- A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair inGenetics · 2017Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Given the frequent misregulation of chromatin in cancer, it is important to understand the cellular mechanisms that regulate chromatin structure. However, systematic screening for epigenetic regulators is challenging and often relies on laborious assays or indirect reporter read-outs. Here we describe a strategy, Epi-ID, to directly assess chromatin status in thousands of mutants. In Epi-ID, chromatin status on DNA barcodes is interrogated by chromatin immunoprecipitation followed by deep sequencing, allowing for quantitative comparison of many mutants in parallel. Screening of a barcoded yeast knock-out collection for regulators of histone H3K79 methylation by Dot1 identified all known regulators as well as novel players and processes. These include histone deposition, homologous recombination, and adenosine kinase, which influences the methionine cycle. Gcn5, the acetyltransferase within the SAGA complex, was found to regulate histone methylation and H2B ubiquitination. The concept of Epi-ID is widely applicable and can be readily applied to other chromatin features.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.