ArticleNature communications2016
Detecting stoichiometry of macromolecular complexes in live cells using FRET.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 77 citations in OpenAlex.
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- Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium Uptake.Journal of cellular physiology · 2025Article
- Investigating genotype-phenotype correlation of limb-girdle muscular dystrophy R8: association of clinical severity, protein biological function and protein oligomerization.Acta neuropathologica communications · 2025Article
- Arrhythmia-associated calmodulin variants interact with KCNQ1 to confer aberrant membrane trafficking and function.PNAS nexus · 2023Article
- Regulation of Cardiac Cav1.2 Channels by Calmodulin.International journal of molecular sciences · 2023Review
- R-type voltage-gated CaCell reports · 2022Article
- NaStructure (London, England : 1993) · 2021Article
- Bak instead of Bax plays a key role in metformin-induced apoptosis s in HCT116 cells.Cell death discovery · 2021Article
- Article
- An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel.BMC biology · 2021Article
- Probing ion channel macromolecular interactions using fluorescence resonance energy transfer.Methods in enzymology · 2021Article
- CaThe Journal of biological chemistry · 2020Article
- Stoichiometry and regulation network of Bcl-2 family complexes quantified by live-cell FRET assay.Cellular and molecular life sciences : CMLS · 2020Article
- Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Quantifying the inhibitory effect of Bcl-xl on the action of Mff using live-cell fluorescence imaging.FEBS open bio · 2019Article
- Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.International journal of molecular sciences · 2019Article
- Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells.Scientific reports · 2019Article
- Single-Channel Resolution of the Interaction between C-Terminal CaBiophysical journal · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The stoichiometry of macromolecular interactions is fundamental to cellular signalling yet challenging to detect from living cells. Fluorescence resonance energy transfer (FRET) is a powerful phenomenon for characterizing close-range interactions whereby a donor fluorophore transfers energy to a closely juxtaposed acceptor. Recognizing that FRET measured from the acceptor's perspective reports a related but distinct quantity versus the donor, we utilize the ratiometric comparison of the two to obtain the stoichiometry of a complex. Applying this principle to the long-standing controversy of calmodulin binding to ion channels, we find a surprising Ca
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Registered trials
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