ArticleNature communications2016
Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Preparation of Plasma Membrane Vesicles, Lysosomes, Proteoliposomes and Alternative Sample Types for SSM-Based Electrophysiology.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases.eLife · 2025Article
- Revealing the glutamate switch: Insights into proton translocation in plant HBiophysical journal · 2025Article
- Article
- NaInternational journal of molecular sciences · 2024Article
- HParasitology research · 2024Review
- Review
- Exploration of the Catalytic Cycle Dynamics of Vigna Radiata HThe Journal of membrane biology · 2023Article
- Spark of Life: Role of Electrotrophy in the Emergence of Life.Life (Basel, Switzerland) · 2023Review
- A self-sustaining serpentinization mega-engine feeds the fougerite nanoengines implicated in the emergence of guided metabolism.Frontiers in microbiology · 2023Article
- Yeast as a tool for membrane protein production and structure determination.FEMS yeast research · 2022Article
- mPPases create a conserved anionic membrane fingerprint as identified via multi-scale simulations.PLoS computational biology · 2022Article
- Pre-steady-state kinetics and solvent isotope effects support the "billiard-type" transport mechanism in NaProtein science : a publication of the Protein Society · 2022Article
- The Mechanism of Energy Coupling in HInternational journal of molecular sciences · 2022Review
- A Lumenal Loop Associated with Catalytic Asymmetry in Plant Vacuolar HInternational journal of molecular sciences · 2021Article
- Exploration of Pyrazolo[1,5-a]pyrimidines as Membrane-Bound Pyrophosphatase Inhibitors.ChemMedChem · 2021Article
- Catalytic Asymmetry in Homodimeric HInternational journal of molecular sciences · 2021Article
- Molecular characterization and transcriptional regulation of two types of HScientific reports · 2021Article
- Discovery of Membrane-Bound Pyrophosphatase Inhibitors Derived from an Isoxazole Fragment.ACS medicinal chemistry letters · 2020Article
- The HFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Membrane-bound pyrophosphatases (M-PPases), which couple proton/sodium ion transport to pyrophosphate synthesis/hydrolysis, are important in abiotic stress resistance and in the infectivity of protozoan parasites. Here, three M-PPase structures in different catalytic states show that closure of the substrate-binding pocket by helices 5-6 affects helix 13 in the dimer interface and causes helix 12 to move down. This springs a 'molecular mousetrap', repositioning a conserved aspartate and activating the nucleophilic water. Corkscrew motion at helices 6 and 16 rearranges the key ionic gate residues and leads to ion pumping. The pumped ion is above the ion gate in one of the ion-bound structures, but below it in the other. Electrometric measurements show a single-turnover event with a non-hydrolysable inhibitor, supporting our model that ion pumping precedes hydrolysis. We propose a complete catalytic cycle for both proton and sodium-pumping M-PPases, and one that also explains the basis for ion specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.