Evidence map›Paper›PMID 27921010›Full record

ArticleFrontiers in cellular and infection microbiology2016

Structural Insights into Substrate Recognition by

Jui-Chieh Yin, Chun-Hsien Fei, Yen-Chen Lo, Yu-Yuan Hsiao, Jyun-Cyuan Chang, Jay C Nix, Yuan-Yu Chang, Lee-Wei Yang, I-Hsiu Huang, Shuying Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Functional analysis ofThe Journal of biological chemistry · 2020
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Jui-Chieh YinDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University Tainan, Taiwan.
Chun-Hsien FeiDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University Tainan, Taiwan.
Yen-Chen LoInstitute of Bioinformatics and Structural Biology, National Tsing Hua UniversityHsinchu, Taiwan; Bioinformatics Program, Taiwan International Graduate Program, Academia SinicaTaipei, Taiwan.
Yu-Yuan HsiaoDepartment of Biological Science and Technology, National Chiao Tung University Hsinchu, Taiwan.
Jyun-Cyuan ChangDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University Tainan, Taiwan.
Jay C NixMolecular Biology Consortium, Advanced Light Source, Lawrence Berkeley National Laboratory Berkeley, CA, USA.
Yuan-Yu ChangInstitute of Bioinformatics and Structural Biology, National Tsing Hua University Hsinchu, Taiwan.
Lee-Wei YangInstitute of Bioinformatics and Structural Biology, National Tsing Hua UniversityHsinchu, Taiwan; Physics Division, National Center for Theoretical SciencesHsinchu, Taiwan.
I-Hsiu HuangDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung UniversityTainan, Taiwan.
Shuying WangDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung UniversityTainan, Taiwan.
National Cheng Kung University · TWAcademia Sinica · TWLawrence Berkeley National Laboratory · USNational Center for Theoretical Sciences · TWNational Tsing Hua University · TWNational Yang Ming Chiao Tung University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sortases function as cysteine transpeptidases that catalyze the covalent attachment of virulence-associated surface proteins into the cell wall peptidoglycan in Gram-positive bacteria. The substrate proteins targeted by sortase enzymes have a cell wall sorting signal (CWSS) located at the C-terminus. Up to date, it is still not well understood how sortases with structural resemblance among different classes and diverse species of bacteria achieve substrate specificity. In this study, we focus on elucidating the molecular basis for specific recognition of peptide substrate PPKTG by

Indexed as

AminoacyltransferasesBacterial ProteinsClostridioides difficileCrystallography, X-RayCysteine EndopeptidasesDNA Mutational AnalysisFluorescence Resonance Energy TransferModels, MolecularMolecular Dynamics SimulationMutagenesis, Site-DirectedPeptidesProtein ConformationSubstrate SpecificityAminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesPeptidessortase BClostridium difficilecrystal structurefluorescence resonance energy transfersortasesubstrate specificity

Identifiers

PMID27921010
PMCPMC5118464
OpenAlexW2552456516

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.