Evidence map›Paper›PMID 27919444›Full record

ArticleDental materials : official publication of the Academy of Dental Materials2017

Mechanistic, genomic and proteomic study on the effects of BisGMA-derived biodegradation product on cariogenic bacteria.

Lida Sadeghinejad, Dennis G Cvitkovitch, Walter L Siqueira, Justin Merritt, J Paul Santerre, Yoav Finer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Dental materials : official publication of the Academy of Dental Materials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
6.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 52 citations in OpenAlex.

  1. Article
  2. Evolution of Dental Resin Adhesives-A Comprehensive Review.Journal of functional biomaterials · 2025
    Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. A novel methacrylate derivative polymer that resists bacterial cell-mediated biodegradation.Journal of biomedical materials research. Part B, Applied biomaterials · 2022
    Article
  9. Article
  10. Antimicrobial antidegradative dental adhesive preserves restoration-tooth bond.Dental materials : official publication of the Academy of Dental Materials · 2020
    Article
  11. Article
  12. Article
  13. Alternative monomer for BisGMA-free resin composites formulations.Dental materials : official publication of the Academy of Dental Materials · 2020
    Article
  14. Article
  15. Article
  16. Threats to adhesive/dentin interfacial integrity and next generation bio-enabled multifunctional adhesives.Journal of biomedical materials research. Part B, Applied biomaterials · 2019
    Review
  17. Esterases affect the physical properties of materials used to seal the endodontic space.Dental materials : official publication of the Academy of Dental Materials · 2019
    Article
  18. Biostable, antidegradative and antimicrobial restorative systems based on host-biomaterials and microbial interactions.Dental materials : official publication of the Academy of Dental Materials · 2019
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Lida SadeghinejadDental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Dennis G CvitkovitchDental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Walter L SiqueiraSchulich Dentistry and Department of Biochemistry, University of Western Ontario, London, Ontario, Canada.
Justin MerrittDepartment of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA.
J Paul SanterreDental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Yoav FinerDental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada. Electronic address: yoav.finer@dentistry.utoronto.ca.
University of Toronto · CAOregon Health & Science University · USWestern University · CA

Funding

The IrvA-Dependent Pathway: A Link Between Stress Adaptation and VirulenceR01DE018893 · NIDCR · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI MERRITT, JUSTIN · 2009 to 2018
$3.0M
New composite material design based on studies of tooth-composite and microbial iR01DE021385 · NIDCR · UNIVERSITY OF TORONTO · PI CVITKOVITCH, DENNIS G., FINER, YOAV · 2010 to 2013
$823k
CIHR MOP115113NIDCR NIH HHS R01 DE018893NIDCR NIH HHS R01 DE021385
6 · The paper itself

Abstract

objectivesInvestigate the effects of a Bis-phenyl-glycidyl-dimethacrylate (BisGMA) biodegradation product, bishydroxypropoxyphenyl-propane (BisHPPP), on gene expression and protein synthesis of cariogenic bacteria.

methodsQuantitative real-time polymerase chain reaction was used to investigate the effects of BisHPPP on the expression of specific virulence-associated genes, i.e. gtfB, gtfC, gbpB, comC, comD, comE and atpH in Streptococcus mutans UA159. Possible mechanisms for bacterial response to BisHPPP were explored using gene knock-out and associated complemented strains of the signal peptide encoding gene, comC. The effects of BisHPPP on global gene and protein expression was analyzed using microarray and quantitative proteomics. The role of BisHPPP in glucosyltransferase (GTF) enzyme activity of S. mutans biofilms was also measured.

resultsBisHPPP (0.01, 0.1mM) up-regulated gtfB/C, gbpB, comCDE, and atpH most pronounced in biofilms at cariogenic pH (5.5). The effects of BisHPPP on the constructed knock-out and complemented strains of comC from quorum-sensing system, implicated this signaling pathway in up-regulation of the virulence-associated genes. Microarray and proteomics identified BisHPPP-regulated genes and proteins involved in biofilm formation, carbohydrate transport, acid tolerance and stress-response. GTF activity was higher in BisHPPP-exposed biofilms when compared to no-BisHPPP conditions. SIGNIFICANCE: These findings provide insight into the genetic and physiological pathways and mechanisms that help explain S. mutans adaptation to restorative conditions that are conducive to increased secondary caries around resin composite restorations and may provide guidance to clinicians' decision on the selection of dental materials when considering the long term oral health of patients and the interactions of composite resins with oral bacteria.

Indexed as

Gene Expression Regulation, BacterialProteomicsBacterial ProteinsBiofilmsBisphenol A-Glycidyl MethacrylateGene ExpressionHumansStreptococcus mutansBacterial ProteinsBisphenol A-Glycidyl MethacrylateBiodegradationBiofilmDental adhesiveGene expressionMicroarrayProteomicsResin compositeSecondary cariesStreptococcus mutans

Identifiers

PMID27919444
PMCPMC5253116
OpenAlexW2558939161

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.