Evidence map›Paper›PMID 27918990›Full record

ArticleJournal of colloid and interface science2017

Effects of tripolyphosphate on cellular uptake and RNA interference efficiency of chitosan-based nanoparticles in Raw 264.7 macrophages.

Bo Xiao, Panpan Ma, Lijun Ma, Qiubing Chen, Xiaoying Si, Lewins Walter, Didier Merlin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of colloid and interface science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Article
  3. Nanoparticles and cytokine response.Frontiers in bioengineering and biotechnology · 2023
    Review
  4. Article
  5. Article
  6. Review
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Bo XiaoInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China; Institute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta 30302, USA. Electronic address: bxiao@gsu.edu.
Panpan MaInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Lijun MaInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Qiubing ChenInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Xiaoying SiInstitute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Lewins WalterInstitute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta 30302, USA.
Didier MerlinInstitute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta 30302, USA; Atlanta Veterans Affairs Medical Center, Decatur 30033, USA.
Southwest University · CNGeorgia State University · US

Funding

ROLE OF CD98 IN INTESTINAL PERMEABILITYR01DK071594 · NIDDK · GEORGIA STATE UNIVERSITY · PI MERLIN, DIDIER · 2006 to 2017
$3.3M
Small intestinal PepT1 expression plays a critical role in maintaining intestinal homeostasis and in shaping the gut microbiotaI01BX002526 · VA · VETERANS HEALTH ADMINISTRATION · PI MERLIN, DIDIER · 2014 to 2024
–
BLRD VA I01 BX002526NIDDK NIH HHS R01 DK071594
6 · The paper itself

Abstract

Tumor necrosis factor-α (TNF-α) is a major pro-inflammatory cytokine that is mainly secreted by macrophages during inflammation. Here, we synthesized a series of N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chlorides (HTCCs), and then used a complex coacervation technique or tripolyphosphate (TPP)-assisted ionotropic gelation strategy to complex the HTCCs with TNF-α siRNA (siTNF) to form nanoparticles (NPs). The resultant NPs had a desirable particle size (210-279nm), a slightly positive zeta potential (14-22mV), and negligible cytotoxicity against Raw 264.7 macrophages and colon-26 cells. Subsequent cellular uptake tests demonstrated that the introduction of TPP to the NPs markedly increased their cellular uptake efficiency (to nearly 100%) compared with TPP-free NPs, and yielded a correspondingly higher intracellular concentration of siRNA. Critically, in vitro gene silencing experiments revealed that all of the TPP-containing NPs showed excellent efficiency in inhibiting the mRNA expression level of TNF-α (by approximately 85-92%, which was much higher than that obtained using Oligofectamine/siTNF complexes). Collectively, these results obviously suggest that our non-toxic TPP-containing chitosan-based NPs can be exploited as efficient siTNF carriers for the treatment of inflammatory diseases.

Indexed as

RNA InterferenceAnimalsChitosanMacrophagesMiceNanoparticlesPolyphosphatesQuaternary Ammonium CompoundsRAW 264.7 CellsRNA, Small InterferingTumor Necrosis Factor-alphaChitosanN-(2-hydroxypropyl)-3-trimethylammonium chitosanPolyphosphatesQuaternary Ammonium CompoundsRNA, Small Interferingtriphosphoric acidTumor Necrosis Factor-alphaChitosanMacrophageN-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chlorideNanoparticleRNA interferenceTripolyphosphate

Identifiers

PMID27918990
PMCPMC5222762
OpenAlexW2549455557

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.