ArticleScientific reports2016
Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 83 citations in OpenAlex.
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- Anther and pollen thermotolerance: molecular mechanisms and implications for plant reproduction under heat stress.Planta · 2026Review
- The Epigenetic Regulation of Agronomic Traits and Environmental Adaptability in Brassicas.Plant, cell & environment · 2025Review
- Plants repress ROS1 expression to attenuate heat-induced transposon burst.Nature plants · 2025Article
- Review
- A chromosome-scale genome assembly and epigenomic profiling reveal temperature-dependent histone methylation in iridoid biosynthesis regulation inHorticulture research · 2025Article
- Effects of thermal interventions on skeletal muscle adaptations and regeneration: perspectives on epigenetics: a narrative review.European journal of applied physiology · 2025Review
- Epigenetic odyssey to decrypt the hidden code for sustainable brassica production: enhancing yield, stress resilience and nutritional quality.Frontiers in plant science · 2025Review
- Article
- Establishment and Maintenance of Heat-Stress Memory in Plants.International journal of molecular sciences · 2024Review
- Plants and global warming: challenges and strategies for a warming world.Plant cell reports · 2024Review
- Changes in epigenetic features in legumes under abiotic stresses.The plant genome · 2023Review
- Response Mechanisms of Woody Plants to High-Temperature Stress.Plants (Basel, Switzerland) · 2023Review
- DNA cytosine methylation dynamics and functional roles in horticultural crops.Horticulture research · 2023Article
- The Change in Whole-Genome Methylation and Transcriptome Profile under Autophagy Defect and Nitrogen Starvation.International journal of molecular sciences · 2023Article
- Review
- Transgressive and parental dominant gene expression and cytosine methylation during seed development in Brassica napus hybrids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- The Chromatin Remodeling FactorPlants (Basel, Switzerland) · 2023Article
- Triticale doubled haploid plant regeneration factors linked by structural equation modeling.Journal of applied genetics · 2022Article
- Integrated methylome and transcriptome analysis unravel the cold tolerance mechanism in winter rapeseed(Brassica napus L.).BMC plant biology · 2022Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 °C for 6 h triggered DNA hypomethylation, particularly in the CG and CHG contexts. And the total number of T32 (Topas 32 °C for 6 h) vs. T0 (Topas 0 h) differentially methylated region-related genes (DRGs) was approximately two-fold higher than that of T18 (Topas 18 °C for 6 h) vs. T0 DRGs, which suggested that 32 °C might be a more intense external stimulus than 18 °C resulting in more changes in the DNA methylation status of cultured microspores. Additionally, 32 °C treatment for 6 h led to increased CHG differential methylations of transposons (DMTs), which were mainly constituted by overlaps between the hypomethylated differentially methylated regions (hypo-DMRs) and transposon elements (TEs). Further analysis demonstrated that the DRGs and their paralogs exhibited differential methylated/demethylated patterns. To summarize, the present study is the first methylome analysis of cultured microspores in response to STHS and may provide valuable information on the roles of DNA methylation in heat response.
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