Evidence map›Paper›PMID 27917838›Full record

ArticleActa crystallographica. Section F, Structural biology communications2016

Overcoming a hemihedral twinning problem in tetrahydrofolate-dependent O-demethylase crystals by the microseeding method.

Ayaka Harada, Yukari Sato, Naofumi Kamimura, Nagarajan Venugopalan, Eiji Masai, Toshiya Senda

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Ayaka HaradaDepartment of Accelerator Science, School of High Energy Accelerator Science, SOKENDAI (The Graduate University for Advanced Studies), 1-1 Oho, Tsukuba, Ibaraki 305-0031, Japan.
Yukari SatoStructural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
Naofumi KamimuraDepartment of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Nagarajan VenugopalanGM/CA@APS, Argonne National Laboratory, Lemont, IL 60439, USA.
Eiji MasaiDepartment of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Toshiya SendaDepartment of Accelerator Science, School of High Energy Accelerator Science, SOKENDAI (The Graduate University for Advanced Studies), 1-1 Oho, Tsukuba, Ibaraki 305-0031, Japan.
High Energy Accelerator Research Organization · JPNagaoka University of Technology · JPArgonne National Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A tetrahydrofolate-dependent O-demethylase, LigM, from Sphingobium sp. SYK-6 was crystallized by the hanging-drop vapour-diffusion method. However, the obtained P3

Indexed as

Amino Acid SequenceBacterial ProteinsCloning, MolecularCrystallizationCrystallography, X-RayEscherichia coliGene ExpressionOxidoreductases, O-DemethylatingPlasmidsRecombinant ProteinsSphingomonadaceaeTetrahydrofolatesX-Ray DiffractionBacterial ProteinsOxidoreductases, O-DemethylatingRecombinant ProteinsTetrahydrofolatescryoprotectanthemihedral twinningmicroseedingO-demethylaseSphingobium sp. SYK-6tetrahydrofolate

Identifiers

PMID27917838
PMCPMC5137467
OpenAlexW2557658376

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.