Evidence map›Paper›PMID 27917835›Full record

ArticleActa crystallographica. Section F, Structural biology communications2016

Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights.

Stavroula Fili, Alexandros Valmas, Magdalini Christopoulou, Maria Spiliopoulou, Nikos Nikolopoulos, Julie Lichière, Souzana Logotheti, Fotini Karavassili, Eleftheria Rosmaraki, Andrew Fitch and 9 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 4 institutions in 3 countries.

Stavroula FiliSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Alexandros ValmasSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Magdalini ChristopoulouSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Maria SpiliopoulouSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Nikos NikolopoulosSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Julie LichièreArchitecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, 13288 Marseille, France.
Souzana LogothetiSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Fotini KaravassiliSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Eleftheria RosmarakiSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
Andrew FitchEuropean Synchrotron Radiation Facility, BP 220, 38043 Grenoble CEDEX 9, France.
Jonathan WrightEuropean Synchrotron Radiation Facility, BP 220, 38043 Grenoble CEDEX 9, France.
Detlef BeckersPANalytical B.V., Lelyweg 1, 7602 EA Almelo, The Netherlands.
Thomas DegenPANalytical B.V., Lelyweg 1, 7602 EA Almelo, The Netherlands.
Gwilherm NénertPANalytical B.V., Lelyweg 1, 7602 EA Almelo, The Netherlands.
Rolf HilgenfeldInstitute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, 23562 Lübeck, Germany.
Nicolas PapageorgiouArchitecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, 13288 Marseille, France.
Bruno CanardArchitecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, 13288 Marseille, France.
Bruno CoutardArchitecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, 13288 Marseille, France.
Irene MargiolakiSection of Genetics, Cell Biology and Development, Department of Biology, University of Patras, University Campus, 26500 Patras, Greece.
University of Patras · GRCentre National de la Recherche Scientifique · FREuropean Synchrotron Radiation Facility · FRUniversity of Lübeck · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viral proteases are proteolytic enzymes that orchestrate the assembly of viral components during the viral life cycle and proliferation. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis are presented of protease 3C, the main protease of an emerging enterovirus, coxsackievirus B3, that is responsible for many cases of viral myocarditis. Polycrystalline protein precipitates suitable for X-ray powder diffraction (XRPD) measurements were produced in the presence of 22-28%(w/v) PEG 4000, 0.1 M Tris-HCl, 0.2 M MgCl

Indexed as

3C Viral ProteasesAmino Acid SequenceCloning, MolecularCrystallizationCrystallography, X-RayCysteine EndopeptidasesEnterovirus B, HumanEscherichia coliGene ExpressionPlasmidsRecombinant ProteinsViral ProteinsX-Ray Diffraction3C Viral ProteasesCysteine EndopeptidasesRecombinant ProteinsViral Proteins3C proteasecoxsackievirus B3powder diffraction

Identifiers

PMID27917835
PMCPMC5137464
OpenAlexW2553077457

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.