Evidence map›Paper›PMID 27916519›Full record

ArticleStructure (London, England : 1993)2017

Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion Channel ELIC.

Qiang Chen, Marta M Wells, Tommy S Tillman, Monica N Kinde, Aina Cohen, Yan Xu, Pei Tang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Qiang ChenDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
Marta M WellsDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA; Department of Computational and System Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Tommy S TillmanDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
Monica N KindeDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
Aina CohenStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Yan XuDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA; Department of Structural Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Pei TangDepartment of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA; Department of Computational and System Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA. Electronic address: ptang@pitt.edu.
University of Pittsburgh · USSLAC National Accelerator Laboratory · US

Funding

X-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6M
ANESTHETIC SITES IN TRANSMEMBRANE PEPTIDES BY NMRR01GM056257 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI TANG, PEI · 1998 to 2017
$4.8M
Integrated Interdisciplinary, inter-university PhD Program Computational Biology (T32)T32EB009403 · NIBIB · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BAHAR, IVET, BAR-JOSEPH, ZIV · 2009 to 2023
$4.4M
ANESTHETIC EFFECTS ON ION CHANNEL STRUCTURES & DYNAMICSR01GM066358 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI TANG, PEI · 2002 to 2014
$3.6M
NMR STUDIES OF MECHANISMS OF GENERAL ANESTHESIAR01GM049202 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI XU, YAN · 2000 to 2018
$3.3M
NIBIB NIH HHS T32 EB009403NIGMS NIH HHS P41 GM103393NIGMS NIH HHS R01 GM049202NIGMS NIH HHS R01 GM056257NIGMS NIH HHS R01 GM066358
6 · The paper itself

Abstract

The structural basis for alcohol modulation of neuronal pentameric ligand-gated ion channels (pLGICs) remains elusive. We determined an inhibitory mechanism of alcohol on the pLGIC Erwinia chrysanthemi (ELIC) through direct binding to the pore. X-ray structures of ELIC co-crystallized with 2-bromoethanol, in both the absence and presence of agonist, reveal 2-bromoethanol binding in the pore near T237(6') and the extracellular domain (ECD) of each subunit at three different locations. Binding to the ECD does not appear to contribute to the inhibitory action of 2-bromoethanol and ethanol as indicated by the same functional responses of wild-type ELIC and mutants. In contrast, the ELIC-α1β3GABA

Indexed as

Binding SitesCrystallography, X-RayDickeya chrysanthemiEthanolHumansLigand-Gated Ion ChannelsModels, MolecularMutationProtein BindingProtein MultimerizationEthanolethylene bromohydrinLigand-Gated Ion ChannelsalcoholsELICethanolpentameric ligand-gated ion channelspore blocking

Identifiers

PMID27916519
PMCPMC5214846
OpenAlexW2557698577

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.