Lidocaine relaxation in isolated rat aortic rings is enhanced by endothelial removal: possible role of K

Aryadi Arsyad, Geoffrey P Dobson

Open access · goldAbstract read

In one paragraph

Article in BMC anesthesiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it

0cells of the map it votes in

4citing papers in PubMed

0.7field-weighted citation impact, top 26% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

Low Calcium-High Magnesium Krebs-Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation.Medicina (Kaunas, Lithuania) · 2024
Article
Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway.Experimental and therapeutic medicine · 2020
Article
Perioperative Use of Intravenous Lidocaine.Drugs · 2018
Review
Adenosine and lidocaine (AL) combination dilates intimally damaged rat thoracic aortic rings and guinea pig mesenteric arteries: possible significance to cardiac surgery.American journal of translational research · 2018
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Aryadi ArsyadHeart, Trauma and Sepsis Research Laboratory, Australian Institute of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, 1 James Cook Drive, 4811, Queensland, Australia.

Geoffrey P DobsonHeart, Trauma and Sepsis Research Laboratory, Australian Institute of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, 1 James Cook Drive, 4811, Queensland, Australia. geoffrey.dobson@jcu.edu.au.

Australian Institute of Tropical Health and Medicine · AUJames Cook University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLidocaine is an approved local anesthetic and Class 1B antiarrhythmic with a number of ancillary properties. Our aim was to investigate lidocaine's vasoreactivity properties in intact versus denuded rat thoracic aortic rings, and the effect of inhibitors of nitric oxide (NO), prostenoids, voltage-dependent K

methodsAortic rings were harvested from adult male Sprague Dawley rats and equilibrated in an organ bath containing oxygenated, modified Krebs-Henseleit solution, pH 7.4, 37 °C. The rings were pre-contracted sub-maximally with 0.3 μM norepinephrine (NE), and the effect of increasing lidocaine concentrations was examined. Rings were tested for viability after each experiment with maximally dilating 100 μM papaverine. The drugs 4-aminopyridine (4-AP), glibenclamide, 5-hydroxydecanoate, ouabain, 8-(3-chlorostyryl) caffeine and PSB-0788 were examined.

resultsAll drugs tested had no significant effect on basal tension. Lidocaine relaxation in intact rings was biphasic between 1 and 10 μM (Phase 1) and 10 and 1000 μM (Phase 2). Mechanical removal of the endothelium resulted in further relaxation, and at lower concentrations ring sensitivity (% relaxation per μM lidocaine) significantly increased 3.5 times compared to intact rings. The relaxing factor(s) responsible for enhancing ring relaxation did not appear to be NO- or prostacyclin-dependent, as L-NAME and indomethacin had little or no effect on intact ring relaxation. In denuded rings, lidocaine relaxation was completely abolished by K

conclusionsWe show that lidocaine relaxation in rat thoracic aorta was biphasic and significantly enhanced by endothelial removal, which did not appear to be NO or prostacyclin dependent. The unknown factor(s) responsible for enhanced relaxation was significantly reduced by K

Indexed as

4-AminopyridineAdenosine A2 Receptor AntagonistsAnimalsAorta, ThoracicCaffeineDecanoic AcidsDose-Response Relationship, DrugEpoprostenolGlyburideHydroxy AcidsIndomethacinIn Vitro TechniquesKATP ChannelsLidocaineMaleNG-Nitroarginine Methyl Ester4-Aminopyridine5-hydroxydecanoic acid8-(3-chlorostyryl)caffeineAdenosine A2 Receptor AntagonistsCaffeineDecanoic AcidsEpoprostenolGlyburideHydroxy AcidsIndomethacinKATP ChannelsLidocaineNG-Nitroarginine Methyl EsterNitric OxideNorepinephrineOuabainPapaverinePotassium Channels, Voltage-GatedReceptor, Adenosine A2AEndotheliumLidocaineNitric oxideRat aortaRedox stressRelaxationVasodilation

Identifiers

PMID27914476

DOI10.1186/s12871-016-0286-y

PMCPMC5135802

OpenAlexW2559339510

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.