ArticlePlant physiology2017
A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 127 citations in OpenAlex.
- Genome-wide analysis of KCS gene family in sunflower reveals the involvement of HaKCS1-1 in wax biosynthesis and drought resistance.BMC plant biology · 2026Article
- Structural, genetic, and adaptive basis of superhydrophobicity in rice leaves.Plant physiology · 2026Article
- The PtrC2H2.2-6-PtrCYP86A7/A8 Module Regulates Poplar Drought Tolerance Through Mediating Cutin and Wax Biosynthesis Pathways.Plant biotechnology journal · 2026Article
- Loss-of-Function Mutation in CER2-LIKE1 Reduced Accumulation of Cuticular Wax and Susceptibility to Thrips in Welsh Onion.Plant biotechnology journal · 2026Article
- Identification of a Leaf Cuticular Wax Biosynthesis GenePlants (Basel, Switzerland) · 2025Article
- Blue-light-enhanced interaction between ZmCRY1s and GL2 modulates epidermal wax composition in Zea mays.Nature communications · 2025Article
- Molecular basis of very-long-chain fatty acid elongation by the CER6-GL2 enzyme complex in plant wax biosynthesis.Science advances · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- Review
- Genome-Wide Analysis ofGenes · 2025Article
- A premature termination codon mutation in the onionHorticulture research · 2025Article
- Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition.The Plant journal : for cell and molecular biology · 2024Article
- Enhancing peanut nutritional quality by editing AhKCS genes lacking natural variation.Plant biotechnology journal · 2024Article
- Broad Chain-Length Specificity of the Alkane-Forming Enzymes NoCER1A and NoCER3A/B in Nymphaea odorata.Plant & cell physiology · 2024Article
- Characterization of the Fatty Acyl-CoA Reductase (FAR) Gene Family and Its Response to Abiotic Stress in Rice (Plants (Basel, Switzerland) · 2024Article
- Transcriptional engineering for value enhancement of oilseed crops: a forward perspective.Frontiers in genome editing · 2024Review
- The impact of the GLOSSY2 and GLOSSY2-LIKE BAHD-proteins in affecting the product profile of the maize fatty acid elongase.Frontiers in plant science · 2024Article
- Cuticular wax in wheat: biosynthesis, genetics, and the stress response.Frontiers in plant science · 2024Review
- Telomere-to-telomere genome assembly of melon (Horticulture research · 2023Article
- OsFAR1 is involved in primary fatty alcohol biosynthesis and promotes drought tolerance in rice.Planta · 2023Article
6 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid elongation and determining substrate specificity. We isolated a rice (Oryza sativa) wax crystal-sparse leaf 4 (WSL4) gene using a map-based cloning strategy. WSL4 is predicted to encode a KCS, a homolog of Arabidopsis (Arabidopsis thaliana) CER6. Complementation of the mutant wsl4-1 with WSL4 genomic DNA rescued the cuticular wax-deficient phenotype, confirming the function of WSL4 The load of wax components longer than 30 carbons (C30) and C28 were reduced markedly in wsl4-1 and wsl4-2 mutants, respectively. Overexpression of WSL4 increased the cuticular wax load in rice leaves. We further isolated a cofactor of WSL4, OsCER2, a homolog of Arabidopsis CER2, by coimmunoprecipitation and confirmed their physical interaction by split-ubiquitin yeast two-hybrid experiments. Expression of WSL4 alone in elo3 yeast cells resulted in increased C24 but did not produce VLCFAs of greater length, whereas expressing OsCER2 alone showed no effect. Coexpression of WSL4 and OsCER2 in elo3 yeast cells yielded fatty acids up to C30. OsCER2 with a mutated HxxxD motif (H172E, D176A, and D176H) interrupted its interaction with WSL4 and failed to elongate VLCFAs past C24 when expressed with WSL4 in elo3 yeast cells. These results demonstrated that WSL4 was involved in VLCFA elongation beyond C22 and that elongation beyond C24 required the participation of OsCER2.
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