ArticleThe Journal of biological chemistry2017
A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Multifunctional lysozymes from the assassin bug Sycanus bifidus: Insecticidal proteins with anticoagulant and melanization inhibition properties.Pest management science · 2026Article
- Host-Associated Differentiation in a Parasitoid Wasp Connected to Venom-Related but Not Chemosensory Genes.Molecular ecology · 2026Article
- Transforming hosts into nurseries and nutrients: strategic manipulation by endoparasitoid wasps.Journal of advanced research · 2026Review
- Review
- The green lacewing venom system and the complex mechanisms underlying its evolution.Molecular biology and evolution · 2026Article
- Ai-Assisted Discovery of a Direct Physical Interaction Between a Venom Serpin from the Parasitoid WaspToxins · 2025Article
- Comprehensive Analysis of Cathepsin Genes in Hemiptera: Functional Characterization of the Venomous Cathepsin B fromInsects · 2025Article
- Effects of Parasitism and Venom from the EndoparasitoidInsects · 2025Article
- Morphotype-Specific Antifungal Defense inInsects · 2025Article
- Innate Immunity in Insects: The Lights and Shadows of Phenoloxidase System Activation.International journal of molecular sciences · 2025Review
- Host and venom evolution in parasitoid wasps: does independently adapting to the same host shape the evolution of the venom gland transcriptome?BMC biology · 2024Article
- Article
- Transcriptomic Insights into Host Metabolism and Immunity Changes after Parasitization byInsects · 2024Article
- Parasitoid Serpins Evolve Novel Functions to Manipulate Host Homeostasis.Molecular biology and evolution · 2023Article
- Larval secretions of parasitoid wasps are new effectors that impair host immune defences.Crop health · 2023Article
- A serpin gene from a parasitoid wasp disrupts host immunity and exhibits adaptive alternative splicing.PLoS pathogens · 2023Article
- Comprehensive isoform-level analysis reveals the contribution of alternative isoforms to venom evolution and repertoire diversity.Genome research · 2023Article
- Host Transcriptome Analysis ofInsects · 2023Article
- Two venom serpins from the parasitoid wasp Microplitis mediator inhibit the host prophenoloxidase activation and antimicrobial peptide synthesis.Insect biochemistry and molecular biology · 2023Article
- The Cellular Immunological Responses and Developmental Differences between Two Hosts Parasitized byLife (Basel, Switzerland) · 2022Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
To ensure successful parasitism, parasitoid wasps inject venom along with their eggs into their hosts. The venom serves to suppress host immune responses, including melanization. Venom from Pteromalus puparum, a pupal endoparasitoid, inhibits melanization of host hemolymph in vitro in a dose-dependent manner. Using assay-guided fractionation, a serpin splicing isoform with phenoloxidase inhibitory activity was identified as P puparum serpin-1, venom isoform (PpS1V). This serpin gene has 16 predicted splicing isoforms that differ only in the C-terminal region. RT-PCR results show that the specific serpin isoform is differentially expressed in the venom gland. Recombinant PpS1V (rPpS1V) suppresses host prophenoloxidase (PPO) activation rather than inhibiting the phenoloxidase directly. Pulldown assays show that PpS1V forms complexes with two host hemolymph proteins, here named Pieris rapae hemolymph proteinase 8 (PrHP8) and P. rapae prophenoloxidase-activating proteinase 1 (PrPAP1), based on gene sequence blasting and phylogenetic analysis. The role of rPrPAP1 in the PPO activation cascade and its interaction with rPpS1V were confirmed. The stoichiometry of inhibition of PrPAP1 by PpS1V is 2.3. PpS1V also inhibits PPO activation in a non-natural host, Ostrinia furnacalis, through forming a complex with O. furnacalis serine protease 13 (OfSP13), an ortholog to PrPAP1. Our results identify a venom-enriched serpin isoform in P. puparum that inhibits host PPO activation, probably by forming a complex with host hemolymph proteinase PrPAP1.
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