ArticleMicrobiome2016
Staphylococcal species heterogeneity in the nasal microbiome following antibiotic prophylaxis revealed by tuf gene deep sequencing.
Article in Microbiome, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
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- Staphylococci in high resolution: Capturing diversity within the human nasal microbiota.Cell reports · 2025Article
- Distinct Intraspecies Variation ofMicroorganisms · 2025Article
- Nanopore-based amplicon sequencing for rapid detection and identification ofFrontiers in microbiology · 2025Article
- The developing pig respiratory microbiome harbors strains antagonistic to common respiratory pathogens.mSystems · 2024Article
- Screening of the novel antimicrobial drug, XF-73, against 2,527Frontiers in cellular and infection microbiology · 2023Article
- Article
- Comparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin.BMC microbiology · 2021Article
- Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-SpecificMicroorganisms · 2020Article
- Assessing similarities and disparities in the skin microbiota between wild and laboratory populations of house mice.The ISME journal · 2020Article
- Application of a High-Throughput Amplicon Sequencing Method to Chart the Bacterial Communities that Are Associated with European Fermented Meats from Different Origins.Foods (Basel, Switzerland) · 2020Article
- Amplicon-Based High-Throughput Sequencing Method Capable of Species-Level Identification of Coagulase-Negative Staphylococci in Diverse Communities.Microorganisms · 2020Article
- Article
- Effect of Co-inhabiting Coagulase Negative Staphylococci onFrontiers in microbiology · 2019Article
- Topographical diversity of common skin microflora and its association with skin environment type: An observational study in Chinese women.Scientific reports · 2017Observational
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundStaphylococci are a major constituent of the nasal microbiome and a frequent cause of hospital-acquired infection. Antibiotic surgical prophylaxis is administered prior to surgery to reduce a patient's risk of postoperative infection. The impact of surgical prophylaxis on the nasal staphylococcal microbiome is largely unknown. Here, we report the species present in the nasal staphylococcal microbiome and the impact of surgical prophylaxis revealed by a novel culture independent technique. Daily nasal samples from 18 hospitalised patients, six of whom received no antibiotics and 12 of whom received antibiotic surgical prophylaxis (flucloxacillin and gentamicin or teicoplanin +/- gentamicin), were analysed by tuf gene fragment amplicon sequencing.
resultsOn admission to hospital, the species diversity of the nasal staphylococcal microbiome varied from patient to patient ranging from 4 to 10 species. Administration of surgical prophylaxis did not substantially alter the diversity of the staphylococcal species present in the nose; however, surgical prophylaxis did impact on the relative abundance of the staphylococcal species present. The dominant staphylococcal species present in all patients on admission was Staphylococcus epidermidis, and antibiotic administration resulted in an increase in species relative abundance. Following surgical prophylaxis, a reduction in the abundance of Staphylococcus aureus was observed in carriers, but not a complete eradication.
conclusionsUtilising the tuf gene fragment has enabled a detailed study of the staphylococcal microbiome in the nose and highlights that although there is no change in the heterogeneity of species present, there are changes in abundance. The sensitivity of the methodology has revealed that the abundance of S. aureus is reduced to a low level by surgical prophylaxis and therefore reduces the potential risk of infection following surgery but also highlights that S. aureus does persist.
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