ArticleProceedings of the National Academy of Sciences of the United States of America2016
Enhanced limonene production in cyanobacteria reveals photosynthesis limitations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 170 citations in OpenAlex.
- Constitutive and inducible oleoresin defenses share genetic architectures and mechanisms in Pinus taeda.The New phytologist · 2026Article
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- Remediation and upcycling of microplastics by algae with wastewater nutrient removal and bioproduction potential.Nature communications · 2025Article
- The Sustainable Production of Terpenoids in Cyanobacterial Chassis.Microorganisms · 2025Review
- Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.NPJ systems biology and applications · 2024Article
- Establishing cell suitability for high-level production of licorice triterpenoids in yeast.Acta pharmaceutica Sinica. B · 2024Article
- The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia.Archives of microbiology · 2024Article
- Application of Cyanobacteria as Chassis Cells in Synthetic Biology.Microorganisms · 2024Review
- Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene inInternational journal of molecular sciences · 2024Article
- Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering.Nature communications · 2023Article
- High salt-induced PSI-supercomplex is associated with high CEF and attenuation of state transitions.Photosynthesis research · 2023Article
- Predicting partner fitness based on spatial structuring in a light-driven microbial community.PLoS computational biology · 2023Article
- Recent developments in the production and utilization of photosynthetic microorganisms for food applications.Heliyon · 2023Review
- Exploring the Potential of the Model CyanobacteriaBiomolecules · 2023Article
- Metabolic network reconstruction ofFrontiers in microbiology · 2023Review
- Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels.Scientific reports · 2022Article
- Article
- Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids inMarine drugs · 2022Article
- Cross-feeding between cyanobacterium Synechococcus and Escherichia coli in an artificial autotrophic-heterotrophic coculture system revealed by integrated omics analysis.Biotechnology for biofuels and bioproducts · 2022Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Terpenes are the major secondary metabolites produced by plants, and have diverse industrial applications as pharmaceuticals, fragrance, solvents, and biofuels. Cyanobacteria are equipped with efficient carbon fixation mechanism, and are ideal cell factories to produce various fuel and chemical products. Past efforts to produce terpenes in photosynthetic organisms have gained only limited success. Here we engineered the cyanobacterium Synechococcus elongatus PCC 7942 to efficiently produce limonene through modeling guided study. Computational modeling of limonene flux in response to photosynthetic output has revealed the downstream terpene synthase as a key metabolic flux-controlling node in the MEP (2-C-methyl-d-erythritol 4-phosphate) pathway-derived terpene biosynthesis. By enhancing the downstream limonene carbon sink, we achieved over 100-fold increase in limonene productivity, in contrast to the marginal increase achieved through stepwise metabolic engineering. The establishment of a strong limonene flux revealed potential synergy between photosynthate output and terpene biosynthesis, leading to enhanced carbon flux into the MEP pathway. Moreover, we show that enhanced limonene flux would lead to NADPH accumulation, and slow down photosynthesis electron flow. Fine-tuning ATP/NADPH toward terpene biosynthesis could be a key parameter to adapt photosynthesis to support biofuel/bioproduct production in cyanobacteria.
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