ArticleThe ISME journal2017
Evolutionary convergence in experimental Pseudomonas populations.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Using TraDIS as a complementary approach to long term evolution for mapping adaptive mutations in Escherichia coli.PLoS genetics · 2026Article
- Genomic and functional analyses reveal Pseudomonas granadensis CT364 is a plant growth-promoting endophyte.BMC microbiology · 2025Article
- Evolutionary rescue of sphericaleLife · 2025Article
- Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens.Environmental microbiology · 2025Article
- Hypermutability bypasses genetic constraints in SCV phenotypic switching in Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2025Article
- An extreme mutational hotspot in nlpD depends on transcriptional induction of rpoS.PLoS genetics · 2025Article
- Extending evolutionary forecasts across bacterial species.Proceedings. Biological sciences · 2024Article
- Genomic sequencing reveals convergent adaptation during experimental evolution in two budding yeast species.Communications biology · 2024Article
- Expanding theFrontiers in microbiology · 2024Article
- Role of extracellular matrix components in biofilm formation and adaptation ofFrontiers in microbiology · 2024Article
- Perspectives on Principles of Cellular Behavior from the Biophysics of Protists.Integrative and comparative biology · 2023Review
- Mutational hotspots lead to robust but suboptimal adaptive outcomes in certain environments.Microbiology (Reading, England) · 2023Article
- Evolution of cyclic di-GMP signalling on a short and long term time scale.Microbiology (Reading, England) · 2023Article
- Distribution of mutation rates challenges evolutionary predictability.Microbiology (Reading, England) · 2023Article
- Towards evolutionary predictions: Current promises and challenges.Evolutionary applications · 2023Review
- Connecting environmental and evolutionary microbiology for the development of new agrobiotechnological tools.Environmental microbiology · 2023Article
- eDNA, Amyloid Fibers and Membrane Vesicles Identified inInternational journal of molecular sciences · 2022Article
- Adaptation to novel spatially-structured environments is driven by the capsule and alters virulence-associated traits.Nature communications · 2022Article
- Molecular Basis of Wrinkled Variants Isolated FromFrontiers in microbiology · 2022Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Model microbial systems provide opportunity to understand the genetic bases of ecological traits, their evolution, regulation and fitness contributions. Experimental populations of Pseudomonas fluorescens rapidly diverge in spatially structured microcosms producing a range of surface-colonising forms. Despite divergent molecular routes, wrinkly spreader (WS) niche specialist types overproduce a cellulosic polymer allowing mat formation at the air-liquid interface and access to oxygen. Given the range of ways by which cells can form mats, such phenotypic parallelism is unexpected. We deleted the cellulose-encoding genes from the ancestral genotype and asked whether this mutant could converge on an alternate phenotypic solution. Two new traits were discovered. The first involved an exopolysaccharide encoded by pgaABCD that functions as cell-cell glue similar to cellulose. The second involved an activator of an amidase (nlpD) that when defective causes cell chaining. Both types form mats, but were less fit in competition with cellulose-based WS types. Surprisingly, diguanylate cyclases linked to cellulose overexpression underpinned evolution of poly-beta-1,6-N-acetyl-d-glucosamine (PGA)-based mats. This prompted genetic analyses of the relationships between the diguanylate cyclases WspR, AwsR and MwsR, and both cellulose and PGA. Our results suggest that c-di-GMP regulatory networks may have been shaped by evolution to accommodate loss and gain of exopolysaccharide modules facilitating adaptation to new environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.